HIV immune reconstitution syndrome in sub-Saharan Africa.
HIV immune reconstitution syndrome in sub-Saharan Africa.
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撒哈拉以南非洲地区的艾滋病毒免疫重建综合症。
DOI：
10.1097/qad.0b013e328308de33
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
VanRie,Annelies
中科院分区：
文献类型：
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作者：
Murdoch,DavidM;Venter,WillemDF;Feldman,Charles;VanRie,Annelies
The various constructs were tested for their ability to activate either LTR-B-mediated or LTR-C-mediated transactivation in human Jurkat T cells as described earlier [8, 9] and the results from three independent experiments (meanÆSD) are shown in Fig. 1. Lane 1 shows the background activity from cells only with LTR-B reporter plasmid DNA; an approximately 17-fold increase with wild-type Tat-B (lane 2); but an additional almost threefold increase with Tat-B construct, which possessed QGD motif in place of RGD (hereafter called Mt-1 construct)(lane 3). The wild-type subtype C construct showed similar activity as obtained with wild-type B construct (compare lanes 2 and 4). The Tat construct possessing wild-type subtype C exon but the RGD motif (found in the second exon of subtype-B, hereafter referred to as Mt-2), showed significant reduction compared with wild-type B or wild-type C constructs. Lanes 6–10 represent the same experiment that was repeated with LTR-C-Luciferase DNA, which essentially gave the same pattern of promoter activation. An exactly similar pattern was observed when the same experiment was carried out on human 293 cells (data not shown).We next evaluated the extent of apoptosis in PMA-stimulated THP-1cells (transformed human macrophages) caused by various wild-type and chimeric (B/C) Tat constructs (indicated at the top of each panel) including the possible role of RGD/QGD motifs only and the representative results from three independent experiments are shown in Fig. 1b. As expected, control cells showed almost 11% apoptosis (panel I); construct with both wild-type Tat-B exons, showed approximately 29% apoptosis (panel II); with Mt-1 construct, it remained the same as with wild-type B (panel III); wild-type Tat-C showed approximately 14%(panel V) apoptosis, which is about 15% less than what was observed with wild-type Tat-B in panel II. Interestingly, in the construct where first exon of Tat-B was fused with second exon of Tat-C, a much reduced cell death was observed (panel IV). The most interesting results were obtained with Mt-2 construct, which showed approximately 10% more cell death (panel VI) when compared with the wild-type Tat-C. A chimeric Tat construct with first exon fused with second exon of Tat-B (both wild type) showed intermediate levels of cell death (panel VII).
