Optimization of extraction of phenolic compounds from wheat using response surface methodology
Optimization of extraction of phenolic compounds from wheat using response surface methodology
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DOI：
10.1016/j.foodchem.2004.08.050
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发表时间：
2005-11-01
期刊：
影响因子：
8.8
通讯作者：
Shahidi, F
中科院分区：
文献类型：
--
作者：
Liyana-Pathirana, C;Shahidi, F
利用响应曲面法确定了从软、硬小麦全粒和麸皮中提取粗酚类物质的最佳工艺条件。采用面心立方设计(FCD)考察了溶剂组成(%)、提取温度(℃)和提取时间(Min)三个自变量对总抗氧化活性(TAA)的影响。对自变量进行了三个水平的编码，并根据初步实验结果选取了自变量的实际值。FCD由14个实验点和中心点的3个重复组成。使用设计专家和统计分析系统软件对数据进行分析。采用二次多项式模型进行预测。回归分析表明，89%以上的变异可以用模型来解释。表面响应的正则分析表明，静止表面是一个鞍形表面。岭分析得到软小麦全粒和麸皮的总氨基酸含量分别为54%、61℃、min和49%、℃、60 min。在最佳条件下，TAA的预测响应值分别为56.5和63TE。为了验证模型的有效性，在最佳条件下提取了粗酚类物质。软质小麦全粒和麸皮的TAA分别为54.7+/-3.2和61.3+/-1.9TE，硬质小麦的TAA也有类似的变化趋势。实验值与预测值吻合较好，表明了模型的适用性和响应面方法在优化提取条件上的成功。(C)2004爱思唯尔有限公司。保留所有权利。
The optimum conditions for the extraction of crude phenolics from whole grain and bran of soft and hard wheat were determined using response surface methodology (RSM). A face-centered cubic design (FCD) was used to investigate the effects of three independent variables, namely solvent composition (%), extraction temperature (degrees C) and time (min) on the response, total antioxidant activity (TAA). The independent variables were coded at three levels and their actual values selected on the basis of preliminary experimental results. The FCD consisted of 14 experimental points and three replications at the center point. Data were analyzed using design expert and statistical analysis system software. A second-order polynomial model was used for predicting the response. Regression analysis showed that more than 89 % of the variation was explained by the models. Canonical analysis of surface responses revealed that the stationary surface was a saddle. The optimal conditions for the TAA obtained using ridge analysis were 54 %, 61 degrees C, 64 min and 49 %, 64 degrees C, 60 min, for whole grain and bran of soft wheat, respectively. Under the optimum conditions the corresponding predicted response values for TAA were 56.5 and 63 TE. The crude phenolics were extracted under optimum conditions to check the validity of the model. The values were 54.7 +/- 3.2 and 61.3 +/- 1.9 TE, for whole grain and bran of soft wheat, respectively; A similar trend was observed for TAA of hard wheat. The experimental values agreed with those predicted, thus indicating suitability of the model employed and the success of RSM in optimizing the extraction conditions. (c) 2004 Elsevier Ltd. All rights reserved.