INFLUENCE OF PH, NUTRIENT AVAILABILITY, AND GROWTH-RATE ON AMINE PRODUCTION BY BACTEROIDES-FRAGILIS AND CLOSTRIDIUM-PERFRINGENS
INFLUENCE OF PH, NUTRIENT AVAILABILITY, AND GROWTH-RATE ON AMINE PRODUCTION BY BACTEROIDES-FRAGILIS AND CLOSTRIDIUM-PERFRINGENS
复制标题
DOI：
10.1128/aem.55.11.2894-2898.1989
复制
发表时间：
1989-11-01
影响因子：
4.4
通讯作者：
MACFARLANE, GT
中科院分区：
文献类型：
--
作者：
ALLISON, C;MACFARLANE, GT
脆弱类杆菌NCDO 2217在分批培养的生长活跃期形成的主要胺有二甲胺、甲胺、丙胺和吡咯烷。这些代谢产物的产生强烈地依赖于pH，并且在酸性条件下(pH 6.0)是最适的。低pH也有利于产气荚膜梭菌C533产生吡咯烷、尸胺和二甲胺，而腐胺、丁胺和丙胺则相反，中性pH时产量最大。脆弱芽孢杆菌在淀粉或酪蛋白限制下连续培养。胺的形成受碳水化合物有效性的影响，当细菌在淀粉限制下以高生长速度(稀释率，0.20/h)生长时，胺的形成最大，约占所测发酵产物的18%。当产气荚膜梭菌在葡萄糖限制的连续培养中生长时，胺的产量是最佳的，并且随着生长速度的增加而增加。在高生长速率和葡萄糖限制的条件下，胺类物质约占所测发酵产物的27%。当饲料中的葡萄糖从5 g/L增加到15 g/L时，胺的产生受到抑制，在这种营养条件下，生长速度对过程几乎没有影响。
Dimethylamine, methylamine, propylamine, and pyrrolidine were the major amines formed by Bacteroides fragilis NCDO 2217 during the active phase of growth in batch culture. Production of these metabolites was strongly pH dependent and was optimal under acidic conditions (pH 6.0). Low pH also favored the formation of pyrrolidine, cadaverine, and dimethylamine by Clostridium perfringens C533, but the reverse was the case with putrescine, butylamine, and propylamine, where production was maximal at neutral pH. B. fragilis was grown in continuous culture under either starch or casein limitation. Amine formation was influenced by carbohydrate availability and was greatest when the bacteria were grown at high growth rates (dilution rate, 0.20/h) under starch limitation, where they constituted about 18% of the total fermentation products measured. Amine production was optimal and increased concomitantly with growth rate when C. perfringens was grown in glucose-limited continuous culture. Under conditions of high growth rate and glucose limitation, amines accounted for approximately 27% of the fermentation products measured. When glucose in the feed medium was increased from 5 to 15 g/liter, amine production was repressed, and under these nutritional conditions the growth rate had little effect on the process.