Cerebroprotective effects of red ginseng extract pretreatment against ischemia-induced oxidative stress and apoptosis
Cerebroprotective effects of red ginseng extract pretreatment against ischemia-induced oxidative stress and apoptosis
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DOI：
10.3109/00207454.2012.758120
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发表时间：
2013-04-01
影响因子：
2.2
通讯作者：
Kim, Gyung Whan
中科院分区：
文献类型：
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作者：
Cheon, So Yeong;Cho, Kyoung Joo;Kim, Gyung Whan
人参C.A.Meyer传统上被用作药用植物，由于其药理作用而具有良好的功效。尽管人参被认为在异常情况下具有保护作用，但红参(RG)提取物预处理对缺血性卒中的影响尚未完全阐明。本实验研究了人参提取物对小鼠局灶性脑缺血的保护作用。RG粗提物(360 mg/kg)腹腔注射，连续2周。小鼠大脑中动脉闭塞1h，再灌流4h和24h。缺血/再灌注(I/R)后给予人参皂苷提取物，缺血区氧化的氢乙啶信号明显减少。免疫荧光染色显示缺血半影区8-羟基鸟苷和凋亡信号调节蛋白1(ASK1)阳性细胞在缺血再灌注后4、24小时明显减少。Western blotting结果显示，RG能有效降低缺血半暗带内激活的ASK1蛋白水平。因此，RG提取物预处理可减少缺血再灌注后24小时的DNA断裂和脑梗塞体积，并且在I/R后的旋转棒试验中表现出更好的表现。因此，RG预处理在抑制缺血诱导的氧化应激和细胞凋亡方面具有保护作用。用RG粗提物预处理可能是预防缺血性卒中后脑损伤的有效策略。
Panax ginseng C.A. Meyer has been traditionally used as a medicinal plant and has beneficial effects due to pharmacological properties. Although ginseng is thought to be protective under abnormal conditions, the effects of pretreatment with red ginseng (RG) extract on ischemic stroke have not been fully elucidated. We investigated the protective effects of RG extract after focal cerebral ischemia in mice. Crude RG extract (360 mg/kg) was administered intraperitoneally for 2 weeks. Mice were then subjected to occlusion of the middle cerebral artery for 1 hour, followed by reperfusion for 4 and 24 hours. Pretreatment with RG extract followed by ischemia/reperfusion (I/R) resulted in significant reduction of oxidized hydroethidine signals in ischemic areas. At 4 and 24 hours after I/R, the number of 8-hydroxyguanosine and apoptosis signal-regulating kinase 1 (ASK1)-positive cells decreased in the ischemic penumbra as seen using immunofluorescent staining. Western blotting showed that RG efficiently attenuated the protein levels of activated ASK1 in the ischemic penumbra. Consequently, DNA fragmentation and the infarct volume were reduced by RG extract pretreatment 24 hours after I/R. Also, RG extract resulted in better performance in rotarod test after I/R. Thus, RG pretreatment demonstrates a protective effect at suppressing ischemia-induced oxidative stress and apoptosis in ischemic lesions. Pretreatment with crude RG extract may be an effective strategy for preventing brain injury after an ischemic stroke.