Proteomics of the organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans adapted to tetrachloroethene and other energy substrates.
Proteomics of the organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans adapted to tetrachloroethene and other energy substrates.
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DOI：
10.1038/srep13794
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发表时间：
2015-09-21
影响因子：
4.6
通讯作者：
Diekert G
中科院分区：
文献类型：
--
作者：
Goris T;Schiffmann CL;Gadkari J;Schubert T;Seifert J;Jehmlich N;von Bergen M;Diekert G
有机卤化物呼吸是一种重要的无氧呼吸，但其特征却很不明确。我们比较了与不同电子受体（富马酸盐，硝酸盐或四氯乙烯[PCE]）生长的多功能有机卤化物呼吸ε-变形杆菌Sulfurophyllum multivorans的全球蛋白质组。有机卤化物呼吸区的基因产物的蛋白质丰度的最显着差异。该基因组区域编码含有PCE还原性脱卤酶PceA和其他参与PCE代谢的蛋白质，例如参与类可啉生物合成的蛋白质的类可啉和FeS簇。后者的基因产物，以及PceA和一个假定的醌醇脱氢酶几乎只检测到与PCE生长的细胞。这一发现表明，电子流从电子供体，如甲酸或丙酮酸通过醌池和醌醇脱氢酶PceA和终端电子受体PCE。两个假定的辅助蛋白，一个ISCU样蛋白和过氧化物酶样蛋白，检测与PCE只有可能参与PceA的成熟。用丙酮酸盐代替甲酸盐作为电子供体生长的细胞的蛋白质组表明电子从还原铁氧还蛋白通过ε-蛋白细菌复合物I和醌池到PCE的路线。
Organohalide respiration is an environmentally important but poorly characterized type of anaerobic respiration. We compared the global proteome of the versatile organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans grown with different electron acceptors (fumarate, nitrate, or tetrachloroethene [PCE]). The most significant differences in protein abundance were found for gene products of the organohalide respiration region. This genomic region encodes the corrinoid and FeS cluster containing PCE reductive dehalogenase PceA and other proteins putatively involved in PCE metabolism such as those involved in corrinoid biosynthesis. The latter gene products as well as PceA and a putative quinol dehydrogenase were almost exclusively detected in cells grown with PCE. This finding suggests an electron flow from the electron donor such as formate or pyruvate via the quinone pool and a quinol dehydrogenase to PceA and the terminal electron acceptor PCE. Two putative accessory proteins, an IscU-like protein and a peroxidase-like protein, were detected with PCE only and might be involved in PceA maturation. The proteome of cells grown with pyruvate instead of formate as electron donor indicates a route of electrons from reduced ferredoxin via an Epsilonproteobacterial complex I and the quinone pool to PCE.