ELECTRO-PHYSIOLOGICAL PROPERTIES OF INVITRO PURKINJE-CELL SOMATA IN MAMMALIAN CEREBELLAR SLICES
ELECTRO-PHYSIOLOGICAL PROPERTIES OF INVITRO PURKINJE-CELL SOMATA IN MAMMALIAN CEREBELLAR SLICES
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DOI：
10.1113/jphysiol.1980.sp013357
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发表时间：
1980-01-01
影响因子：
5.5
通讯作者：
SUGIMORI, M
中科院分区：
文献类型：
--
作者：
LLINAS, R;SUGIMORI, M
在豚鼠小脑片上研究了浦肯野细胞的电活动。在直视下获得浦肯野细胞体细胞内的记录。表现出逆行、突触和直接电反应。膜直接去极化可逆转爬行纤维传入所产生的突触电位。浦肯野细胞在胞体表现出三种类型的电压依赖性电反应：快速钠依赖峰，非钝化钠电导，产生长时间的平台，平台的幅度由电压依赖的钠和钾电导之间的平衡产生，钙依赖的尖峰是河豚毒素不敏感的，但对阻止慢钙电导的物质有反应。Ca峰在树突中最为突出，其次是K电导的持续变化。这种钙和钾的活性是浦肯野细胞长期兴奋性中最重要的自我调节机制。在没有钙电导的情况下，浦肯野细胞的行为不稳定，产生长时间和无调控的高频放电。
The electrical activity of Purkinje cells was studied in guinea-pig cerebellar slices in vitro. Intracellular recordings from Purkinje cell somata were obtained under direct vision. Antidromic, synaptic and direct electroresponsiveness was demonstrated. Synaptic potentials produced by the activation of the climbing fiber afferent could be reversed by direct membrane depolarization. Purkinje cells demonstrated 3 types of voltage-dependent electroresponsiveness at the soma: a fast Na-dependent spike, a noninactivating Na conductance which generated prolonged plateaus, where the amplitude of the plateau was produced by an equilibrium between the voltage-dependent Na and K conductances and Ca-dependent spiking which was TTX[tetrodotoxin]-insensitive but responds to substances blocking slow Ca conductances. The Ca spikes were most prominent in the dendrites and were followed by prolonged K conductance changes. This Ca and K activity was the most significant self-regulatory mechanism in long-term excitability of Purkinje cells. In the absence of Ca conductance, Purkinje cells behaved in an erratic fashion, producing prolonged and unregulated periods of high frequency firing.