Genotypic diversity of the cotton-melon aphid Aphis gossypii (Glover) in Tunisia is structured by host plants
Genotypic diversity of the cotton-melon aphid Aphis gossypii (Glover) in Tunisia is structured by host plants
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DOI：
10.1017/s0007485307005585
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发表时间：
2008-08-01
影响因子：
1.9
通讯作者：
Vanlerberghe-Masutti, F.
中科院分区：
文献类型：
--
作者：
Charaabi, K.;Carletto, J.;Vanlerberghe-Masutti, F.
研究多食性昆虫在寄主植物利用方面的种内变异对于理解昆虫-植物相互作用的进化模式至关重要。棉蚜（Aphis gossypii，格洛弗）是世界性的广食性蚜虫。如果寄主植物种类或科对这些蚜虫构成选择性制度，则可能发生遗传分化和与寄主相关的适应。在这项研究中，我们描述了A。在突尼斯的六个地方收集的不同蔬菜作物、柑橘树和木槿上的棉蚜。目的是确定蚜虫种群的结构是否与宿主植物有关，以及这种分化是否在不同地点之间一致。对A.在8个微卫星位点上检测了棉种样品。在559个个体中，我们只发现了11个多位点基因型。显著偏离Hardy-Weinberg平衡，连锁不平衡和缺乏重组基因型，证实了A。棉株的生殖方式为连续的孤雌生殖。不同寄主植物家系间的遗传分化较强（0.1.75 < F（ST）< 0.691），而不同寄主植物家系间的遗传分化不明显。绝大多数蚜虫表现出3种优势多位点基因型之一，这3种基因型分别与葫芦科（Cucurbitaceae）、茄科（Solanaceae）和芸香科（Rutaceae）3个植物科重复相关，表明蚜虫的寄主专化性在葫芦科（Cucurbitaceae）、茄科（Solanaceae）和芸香科（Rutaceae）。棉。这些专门的基因型同时发现与其他克隆木槿，这表明这种多年生主机可以作为一个避难所植物之间的两个蔬菜作物季节。
The study of intraspecific variation with respect to host plant utilization in polyphagous insects is crucial for understanding evolutionary patterns of insect-plant interactions. Aphis gossypii (Glover) is a cosmopolitan and extremely polyphagous aphid species. If host plant species or families constitute selective regimes to these aphids, genetic differentiation and host associated adaptation may occur. In this study, we describe the genetic structure of A. gossypii collected in six localities in Tunisia on different vegetable crops, on citrus trees and on Hibiscus. The aim was to determine if the aphid populations are structured in relation to the host plants and if such differentiation is consistent among localities. The genetic variability of A. gossypii samples was examined at eight microsatellite loci. We identified only 11multilocus genotypes among 559 individuals. Significant deviations from Hardy-Weinberg equilibrium, linkage disequilibria and absence of recombinant genotypes, confirmed that A. gossypii reproduces by continuous apomictic parthenogenesis. Genetic differentiation between localities was not significant, whereas a strong differentiation was observed between host plant families (0.1.75 < F(ST) < 0.691). The great majority of aphids exhibited one of three predominant multilocus genotypes that were repeatedly and respectively associated to the three plant families, Cucurbitaceae, Solanaceae and Rutaceae, demonstrating host specialization in A. gossypii. These specialized genotypes were simultaneously found with other clones on Hibiscus, suggesting that this perennial host could act as a refuge plant between two vegetable crop seasons.