ChpA Controls Twitching Motility and Broadly Affects Gene Expression in the Biological Control Agent Lysobacter enzymogenes
ChpA Controls Twitching Motility and Broadly Affects Gene Expression in the Biological Control Agent Lysobacter enzymogenes
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DOI：
10.1007/s00284-017-1202-5
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发表时间：
2017-03
影响因子：
2.6
通讯作者：
Mimi Zhou;D. Shen;Gaoge Xu;Fengquan Liu;Guoliang Qian
中科院分区：
文献类型：
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作者：
Mimi Zhou;D. Shen;Gaoge Xu;Fengquan Liu;Guoliang Qian
产酶溶杆菌（Lysobacter enzymogenes L.）产酶菌（enzymogenes）是农业上重要的革兰氏阴性细菌，其利用T4 P（IV型皮利）驱动的抽搐运动来表现其抗真菌功能。然而，目前还不清楚这种细菌如何调节其抽搐运动。在这里，通过使用菌株OH 11作为工作模式生物，我们表明，一个混合的双组分系统ChpA作为一个积极的调节器控制抽搐运动在L。酶基因ChpA是一种混合TCS（双组分转导系统），包含7个结构域，包括用于自磷酸化和磷酸基团转移的结构域，以及磷酸受体（REC）结构域。突变的chpA完全废除了野生型抽搐运动，证明了移动的细胞在突变集落的边缘的情况下。结构域缺失和表型特征的进一步研究表明，负责磷酸化和磷酸转移的结构域，但不是REC结构域，是必不可少的ChpA在调节抽搐运动。对chpA基因敲除菌株的转录组分析表明，ChpA广泛参与控制多种基因（总计243个）的表达。这些差异表达基因的产物参与了L.酶基因因此，我们不仅确定了一个新的调节器控制抽搐运动的L。ChpA基因的研究，但也提供了第一份报告，证明了保守的ChpA在革兰氏阴性菌的基因调控中的广泛影响。
Lysobacter enzymogenes (L. enzymogenes) is an agriculturally important Gram-negative bacterium that employs T4P (type IV pili)-driven twitching motility to exhibit its antifungal function. Yet, it is still unclear how this bacterium regulates its twitching motility. Here, by using strain OH11 as the working model organism, we showed that a hybrid two-component system ChpA acts as a positive regulator in controlling twitching motility in L. enzymogenes. ChpA is a hybrid TCS (two-component transduction system) contains 7 domains including those for auto-phosphorylation and phosphate group transfer, as well as a phosphate receiver (REC) domain. Mutation of chpA completely abolished the wild-type twitching motility, as evidenced by the absence of mobile cells at the margin of the mutant colonies. Further studies of domain-deletion and phenotypic characterization reveal that domains responsible for phosphorylation and phosphotransfer, but not the REC domain, were indispensable for ChpA in regulating twitching motility. Transcriptome analyses of the chpA knockout strain indicated that ChpA was extensively involved in controlling expression of a wide variety of genes (totaling 243). The products of these differentially expressed genes were involved in multiple physiological and biological functions in L. enzymogenes. Thus, we have not only identified a new regulator controlling twitching motility in L. enzymogenes, but also provided the first report demonstrating the broad impact of the conserved ChpA in gene regulation in Gram-negative bacteria.