Combinatorial RNAi:: a method for evaluating the functions of gene families in Drosophila
Combinatorial RNAi:: a method for evaluating the functions of gene families in Drosophila
复制标题
DOI：
10.1016/s0166-2236(02)02034-9
复制
发表时间：
2002-02-01
影响因子：
15.9
通讯作者：
Zinn, K
中科院分区：
文献类型：
--
作者：
Schmid, A;Schindelholz, B;Zinn, K
基因家族的个别成员在神经系统发育过程中往往具有部分冗余功能，这使得传统的遗传分析存在问题。在这里，我们回顾实验表明，几个基因可以沉默一起注射到野生型果蝇胚胎的双链RNA。通过染料标记单个神经母细胞在注射胚胎，多基因沉默对个别中枢神经系统轴突通路的影响，现在可以检查。
Individual members of gene families often have partially redundant functions during nervous system development, making conventional genetic analysis problematic. Here we review experiments showing that several genes can be silenced together by injection of double-stranded RNAs into wild-type Drosophila embryos. By dye-labeling single neuroblasts in injected embryos, the effects of multigene silencing on individual CNS axon pathways can now be examined.