Glycine intake decreases plasma free fatty acids, adipose cell size, and blood pressure in sucrose-fed rats
Glycine intake decreases plasma free fatty acids, adipose cell size, and blood pressure in sucrose-fed rats
复制标题
DOI：
10.1152/ajpregu.00159.2004
复制
发表时间：
2004-12-01
影响因子：
2.8
通讯作者：
Baños, G
中科院分区：
文献类型：
--
作者：
El Hafidi, M;Pérez, I;Baños, G
本研究探讨了甘氨酸对雄性Wistar大鼠因摄入蔗糖而引起的循环非酯化脂肪酸（NEFA）、脂肪细胞大小、腹腔内脂肪堆积和血压（BP）增加的保护机制。在含有30%蔗糖的饮用水中添加1%甘氨酸4周后，蔗糖喂养大鼠（SFR）的高血压明显降低（122.3 +/- 5.6 vs.不添加甘氨酸的SFR的147.6 +/- 5.4 mmHg, P < 0.001）。甘氨酸治疗组血浆甘油三酯（TG）水平（0.9 +/- 0.3 mM vs. 1.4 +/- 0.3 mM, P < 0.001）、腹内脂肪（6.8 +/- 2.16 g vs. 14.8 +/- 4.0 g, P < 0.01）和脂肪细胞大小均低于未治疗组。甘氨酸摄入显著降低SFR患者血浆NEFA总浓度（0.64 +/- 0.08 mM vs.未摄入甘氨酸的SFR患者血浆NEFA总浓度为1.11 +/- 0.09 mM, P < 0.001）。在对照动物中，甘氨酸降低了葡萄糖、tg和总NEFA，但没有达到显著性。在甘氨酸处理的SFR中，线粒体呼吸作为脂肪氧化速率的指标，显示β -氧化底物辛酸和棕榈酰肉碱的IV态氧化速率增加。这表明肝脏脂肪酸代谢的增强，即，在他们的运输，活化，或β -氧化。这些发现表明，甘氨酸对血压升高的保护作用可能是由于其增加脂肪酸氧化、减少腹部脂肪积累和循环NEFA的作用，而这些作用被认为是肥胖和高血压之间的联系。
The study investigated the mechanism by which glycine protects against increased circulating nonesterified fatty acids ( NEFA), fat cell size, intra- abdominal fat accumulation, and blood pressure ( BP) induced in male Wistar rats by sucrose ingestion. The addition of 1% glycine to the drinking water containing 30% sucrose, for 4 wk, markedly reduced high BP in sucrose- fed rats ( SFR) ( 122.3 +/- 5.6 vs. 147.6 +/- 5.4 mmHg in SFR without glycine, P < 0.001). Decreases in plasma triglyceride ( TG) levels ( 0.9 +/- 0.3 vs. 1.4 +/- 0.3 mM, P < 0.001), intra- abdominal fat ( 6.8 +/- 2.16 vs. 14.8 +/- 4.0 g, P < 0.01), and adipose cell size were observed in SFR treated with glycine compared with SFR without treatment. Total NEFA concentration in the plasma of SFR was significantly decreased by glycine intake ( 0.64 +/- 0.08 vs. 1.11 +/- 0.09 mM in SFR without glycine, P < 0.001). In control animals, glycine decreased glucose, TGs, and total NEFA but without reaching significance. In SFR treated with glycine, mitochondrial respiration, as an indicator of the rate of fat oxidation, showed an increase in the state IV oxidation rate of the beta- oxidation substrates octanoic acid and palmitoyl carnitine. This suggests an enhancement of hepatic fatty acid metabolism, i. e., in their transport, activation, or beta- oxidation. These findings imply that the protection by glycine against elevated BP might be attributed to its effect in increasing fatty acid oxidation, reducing intra- abdominal fat accumulation and circulating NEFA, which have been proposed as links between obesity and hypertension.