Mapping of the Sex Determining Region on Linkage Group 12 of Guppy (Poecilia reticulata)
Mapping of the Sex Determining Region on Linkage Group 12 of Guppy (Poecilia reticulata)
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DOI：
10.1534/g3.119.400656
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发表时间：
2019-11-01
影响因子：
2.6
通讯作者：
Ron, Micha
中科院分区：
文献类型：
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作者：
Dor, Lior;Shirak, Andrey;Ron, Micha
孔雀鱼是最受欢迎的观赏鱼类之一，由于其巨大的彩色鳍，对雄性的需求较高。本研究的目的是定位孔雀鱼第12连锁群的性别决定（SD）区域，并鉴定性别特异性标记。我们产生了8个多态性微卫星标记分布沿着远端5.4 Mbp序列的先前确定的SD区域上的连锁群（LG）12。在156个个体的红金发和火焰株，和126个后代的四个全同胞红金发家庭的标记进行了测试与性别的关联。在LG 12上的25.3 Mbp处发现了一个男性特异性等位基因gu 1066，在Flame株上的24.4 Mbp处发现了一个男性特异性等位基因gu 832。因此，选择这些标记之间的0.9Mbp区域进行分析，该区域包含27个注释基因。基于拷贝数变异和性别决定的关联，我们在LG 12上定位了LG 9和LG 12之间的重复区域，长度为1.26 Mbp，包含59个基因。在以gu 1066和gu 832为界的片段与LG 12上0.43 Mbp的重复区之间的共同区间内，共有11个基因，其中6个基因具有多拷贝（54%）。GADD 45 G-like基因是一个可能的位置和功能候选基因，它在男性生育中起着重要作用。我们的特点是在孔雀鱼的基因组结构的基因，并发现两种异构体，但没有性别偏见的差异是明显的基因组序列和基因表达。
Poecilia reticulata is one of the most popular ornamental fish species with a higher demand for males due to their large colorful fins. The objectives of this study were mapping of the sex-determining (SD) region on linkage group 12 of guppy, and identification of a sex specific marker. We generated eight polymorphic microsatellite markers distributed along the distal 5.4 Mbp sequence of the previously identified SD region on linkage group (LG) 12. The markers were tested for association with sex in 156 individuals of the Red Blonde and Flame strains, and 126 progeny of four full-sibs Red Blonde families. A male-specific allele was found for microsatellite gu1066 at position of 25.3 Mbp on LG12 for both strains, and gu832 at position 24.4 Mbp for the Flame strain. Thus, a region of 0.9 Mbp between these markers, harboring 27 annotated genes, was selected for analysis. Based on association of copy number variation and sex determination we mapped a duplicated region between LGs 9 and 12, of 1.26 Mbp, containing 59 genes on LG12. The common interval between the segment bounded by gu1066 and gu832, and the duplicated region of 0.43 Mbp on LG12 harbors 11 genes of which 6 have multiple copies (54%). Growth arrest and DNA damage inducible gamma-like (GADD45G-like) is a plausible positional and functional candidate gene for its role in male fertility. We characterized the genomic structure of the gene in guppy, and found two isoforms; but no sex-biased differences were evident in genomic sequence and gene expression.