Ectopic expression of angiopoietin-1 promotes neuronal differentiation in neural progenitor cells through the Akt pathway
Ectopic expression of angiopoietin-1 promotes neuronal differentiation in neural progenitor cells through the Akt pathway
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血管生成素-1 的异位表达通过 Akt 途径促进神经祖细胞的神经元分化
DOI：
10.1016/j.bbrc.2008.11.052
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发表时间：
2009-01-09
影响因子：
3.1
通讯作者：
Xiao, Junjun
中科院分区：
文献类型：
--
作者：
Bai, Yun;Cui, Ming;Xiao, Junjun
在成人神经发生的区域，神经祖细胞（NPC）被发现在所谓的“血管龛”内靠近血管。神经发生通过某些生长因子与血管生成相关。我们建议，血管生成素-1（Ang 1），这是类似于VEGF，有一个独特的作用，独立于其在血管生成的神经发生。本研究用表达Ang 1的重组腺病毒转导原代培养的NPC，并用二丁酰环腺苷酸（dbcAMP）诱导分化。通过定量PCR、免疫荧光显微镜和Western印迹分析评价神经元分化。结果表明，异位表达的Ang 1促进神经元分化和轴突生长的NPC，而这种效果被阻断的存在下，抗Tie 2受体抗体或P13-K抑制剂，LY 294002。我们的研究结果表明，Ang 1，最初被确定为血管生成因子，也可以通过Akt途径刺激NPC的体外神经发生。(c)2008年爱思唯尔公司所有的战斗保留。
In regions of adult neurogenesis, neural progenitor cells (NPCs) are found in close proximity to blood vessels within a so-called 'vascular niche'. Neurogenesis is linked to angiogenesis via certain growth factors. We propose that angiopoietin-1 (Ang1), which is similar to VEGF, has a unique role in neurogenesis independent of its role in angiogenesis. In this study, primary cultures of NPCs were transduced with recombinant: adenoviruses expressing Ang1 and induced to differentiate With dibutyryl cyclic AMP (dbcAMP). Neuronal differentiation was evaluated by quantitative PCR, immunofluorescence microscopy and Western blot analysis. The results show that ectopic expression of Ang1 promotes neuronal differentiation and neurite outgrowth in NPCs, while this effect was blocked by the presence of anti-Tie2 receptor antibody or the P13-K inhibitor, LY294002. Our results Suggest that Ang1, identified originally as in angiogenic factor, can also stimulate in vitro neurogenesis in NPCs through the Akt pathway. (c) 2008 Elsevier Inc. All fights reserved.