Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences

Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences
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DOI:
10.1023/a:1001761008817
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发表时间:
1998-05-01
影响因子:
2.6
通讯作者:
Robnett, CJ
Robnett, CJ
中科院分区:
生物学3区
文献类型:
--
作者:
Kurtzman, CP;Robnett, CJ

文献摘要

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大约500种子囊酵母,包括念珠菌和其他变形属的成员,分析了大亚基(26 S)核糖体DNA的可变D1/D2结构域的分歧程度。在这一领域的分歧一般足以解决个别物种,导致预测,目前公认的55个分类群是早期描述的物种的同义词。从D1/D2序列差异分析了子囊酵母的系统发育关系。为了进行比较,根据18 S rDNA序列确定了酵母菌分支选定成员的系统发育关系。种间关系是高度一致的D1/D2和18 S树的分支时,统计上得到很好的支持。
Approximately 500 species of ascomycetous yeasts, including members of Candida and other anamorphic genera, were analyzed for extent of divergence in the variable D1/D2 domain of large subunit (26S) ribosomal DNA. Divergence in this domain is generally sufficient to resolve individual species, resulting in the prediction that 55 currently recognized taxa are synonyms of earlier described species. Phylogenetic relationships among the ascomycetous yeasts were analyzed from D1/D2 sequence divergence. For comparison, the phylogeny of selected members of the Saccharomyces clade was determined from 18S rDNA sequences. Species relationships were highly concordant between the D1/D2 and 18S trees when branches were statistically well supported.