Molecular cloning and expression of a Toll receptor in the giant tiger shrimp, Penaeus monodon
Molecular cloning and expression of a Toll receptor in the giant tiger shrimp, Penaeus monodon
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DOI：
10.1016/j.fsi.2006.08.018
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发表时间：
2007-09-01
影响因子：
4.7
通讯作者：
Stet, Rene J. M.
中科院分区：
文献类型：
--
作者：
Arts, Joop A. J.;Cornelissen, Ferry H. J.;Stet, Rene J. M.
无脊椎动物完全依靠先天免疫系统来抵抗病原体。这种非自我识别被具有独特保守分子模式的微生物细胞壁组分激活。病原体相关分子模式（PAMP）由模式识别受体（PRR）识别。Toll及其哺乳动物同源物Toll样受体是细胞表面受体，充当PRR并参与与其免疫应答有关的信号传导途径。在这里，我们描述了一个新的部分Toll受体基因克隆从鳃库的巨型虎虾，斑节对虾，使用引物的高度保守的Toll/IL-1 R（TIR）结构域的基础上。推测的氨基酸序列与意大利蜜蜂Toll相关蛋白的同源性为59%。对虾Toll的LRR分析不包含明显的PAMP结合插入。与昆虫Toll家族的系统发育分析表明，与Toll 1和Toll 5基因产物聚为一簇，与Toll 3和Toll 4的相关性较小。RT-qPCR结果表明，PmToll在肠道、鳃和肝胰腺中组成型表达。用白色斑点综合征病毒（WSSV）攻击显示在这些器官中表达水平相等。在防御机制中的作用进行了讨论。总之，对虾至少具有一个可能参与免疫防御的Toll受体。(c)2006爱思唯尔有限公司保留所有权利。
Invertebrates rely completely for their protection against pathogens on the innate immune system. This non-self-recognition is activated by microbial cell wall components with unique conserved molecular patterns. Pathogen-associated molecular patterns (PAMPs) are recognised by pattern recognition receptors (PRRs). Toll and its mammalian homologs Toll-like receptors are cell-surface receptors acting as PRRs and involved in the signalling pathway implicated in their immune response. Here we describe a novel partial Toll receptor gene cloned from a gill library of the giant tiger shrimp, Penaeus monodon, using primers based on the highly conserved Toll/IL-lR (TIR) domain. The deduced amino acid sequence of the P. monodon Toll (PmToll) shows 59% similarity to a Toll-related protein of Apis mellifera. Analysis of the LRRs of shrimp Toll contained no obvious PAMP-binding insertions. Phylogenctic analysis with the insect Toll family shows clustering with Toll1 and Toll5 gene products, and it is less related to Toll3 and Toll4. Furthermore, RT-qPCR shows that PmToll is constitutively expressed in gut, gill and hepatopancreas. Challenge with white spot syndrome virus (WSSV) shows equal levels of expression in these organs. A role in the defence mechanism is discussed. In conclusion, shrimp possess at least one Toll receptor that might be involved in immune defence. (c) 2006 Elsevier Ltd. All rights reserved.