Minimal nucleotide duplex formation in water through enclathration in self-assembled hosts
Minimal nucleotide duplex formation in water through enclathration in self-assembled hosts
复制标题
DOI：
10.1038/nchem.100
复制
发表时间：
2009-04-01
期刊：
影响因子：
21.8
通讯作者：
Fujita, Makoto
中科院分区：
文献类型：
--
作者：
Sawada, Tomohisa;Yoshizawa, Michito;Fujita, Makoto
短核苷酸片段例如单核苷酸和二核苷酸通常不能在水中形成稳定的氢键碱基对或双链体。然而，在酶的疏水口袋内，即使是短片段也会形成稳定的双链体来传递遗传信息。在这里，我们证明了通过自组装笼的疏水空腔中的包合，从水中的单核苷酸有效形成氢键碱基对。晶体学研究以及 H-1- 和 N-15-NMR 光谱清楚地揭示了单核苷酸的对选择性识别以及笼腔中反 Hoogsteen 型碱基对的选择性形成。在类似的扩展笼内，还发现二核苷酸在水中形成稳定的双链体。这些结果强调了氢键碱基配对如何在与水溶液隔离的局部疏水区域中被放大。
Short nucleotide fragments such as mono- and dinucleotides are generally unable to form stable hydrogen-bonded base pairs or duplexes in water. Within the hydrophobic pockets of enzymes, however, even short fragments form stable duplexes to transmit genetic information. Here, we demonstrate the efficient formation of hydrogen-bonded base pairs from mononucleotides in water through enclathration in the hydrophobic cavities of self-assembled cages. Crystallographic studies and H-1- and N-15-NMR spectroscopy clearly reveals pair-selective recognition of mononucleotides and the selective formation of an anti-Hoogsteen-type base pair in the cage's cavity. Within an analogous expanded cage, dinucleotides are also found to form a stable duplex in water. These results emphasize how hydrogen-bonded base pairing is amplified in a local hydrophobic area isolated from aqueous solution.