Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis.
Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis.
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DOI：
10.1038/ncomms8640
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发表时间：
2015-07-17
影响因子：
16.6
通讯作者：
Wirtz M
中科院分区：
文献类型：
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作者：
Linster E;Stephan I;Bienvenut WV;Maple-Grødem J;Myklebust LM;Huber M;Reichelt M;Sticht C;Møller SG;Meinnel T;Arnesen T;Giglione C;Hell R;Wirtz M
N-末端乙酰基转移酶（Nats）催化的N-末端乙酰化（NTA）是真核生物中最常见的蛋白质修饰之一，但其意义至今仍是个谜。在这里，我们的植物NatA复杂的特点，并揭示进化保守的NatA生化特性在高等真核生物和揭示特定的和必要的功能NatA的发展，生物合成途径和胁迫反应在植物中。我们发现，NTA减少干旱胁迫后显着，和NatA丰度迅速下调的植物激素脱落酸。因此，转基因下调NatA诱导干旱胁迫反应，并导致显着的抗旱性植物。因此，我们建议，NTA的NatA复合物作为一个细胞的监视机制，在压力和印记的蛋白质组的NatA是一个重要的开关，控制脱落酸下游的代谢，发展和细胞应激反应。 N-末端乙酰化是真核生物中常见的蛋白质修饰。在这里，作者表明，在拟南芥中，N-末端乙酰化减少干旱胁迫，N-末端乙酰转移酶的丰度减少脱落酸和组成性下调可以赋予抗旱性。
N-terminal acetylation (NTA) catalysed by N-terminal acetyltransferases (Nats) is among the most common protein modifications in eukaryotes, but its significance is still enigmatic. Here we characterize the plant NatA complex and reveal evolutionary conservation of NatA biochemical properties in higher eukaryotes and uncover specific and essential functions of NatA for development, biosynthetic pathways and stress responses in plants. We show that NTA decreases significantly after drought stress, and NatA abundance is rapidly downregulated by the phytohormone abscisic acid. Accordingly, transgenic downregulation of NatA induces the drought stress response and results in strikingly drought resistant plants. Thus, we propose that NTA by the NatA complex acts as a cellular surveillance mechanism during stress and that imprinting of the proteome by NatA is an important switch for the control of metabolism, development and cellular stress responses downstream of abscisic acid. N-terminal acetylation is a common protein modification in eukaryotes. Here the authors show that in Arabidopsis, N-terminal acetylation is decreased by drought stress, that abundance of an N-terminal acetyltransferase is reduced by abscisic acid and that constitutive downregulation can confer drought resistance.