An LDLa domain-containing C-type lectin is involved in the innate immunity of Eriocheir sinensis
An LDLa domain-containing C-type lectin is involved in the innate immunity of Eriocheir sinensis
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含有LDLa结构域的C型凝集素参与中华绒螯蟹的先天免疫。
DOI：
10.1016/j.dci.2013.10.004
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发表时间：
2014-01-01
影响因子：
2.9
通讯作者：
Wang, Wen
中科院分区：
文献类型：
--
作者：
Huang, Ying;An, Liang;Wang, Wen
C型凝集素（C-type lectin，CTL）在天然免疫中具有识别和清除病原体的重要功能。本研究从中华绒螯蟹中鉴定了一种新的含LDLa结构域的CTL，命名为EsCTLDcp。EsCTLDcp cDNA全长1258 bp，开放阅读框975 bp，编码324个氨基酸。EsCTLDcp含有信号肽、LDLa和单个C型凝集素样结构域。EsCTLDcp仅在正常蟹的肝胰腺中表达，并且其表达在蟹挑战病原体相关分子模式和细菌后受到调节。重组EsCTLDcp在钙存在下凝集革兰氏阳性菌（金黄色葡萄球菌）和革兰氏阴性菌（副溶血性弧菌和嗜水气单胞菌）。rEsCTLDcp还与包括S.金黄色葡萄球菌、苏云金芽孢杆菌、枯草芽孢杆菌、大肠杆菌、产钠弧菌、副溶血性弧菌和A.你好rEsCTLDcp蛋白帮助螃蟹在体内清除毒性革兰氏阴性细菌副溶血性弧菌，并与白色斑点综合征病毒（WSSV）的包膜蛋白VP 24相互作用。这些数据表明EsCTLDcp作为模式识别受体参与E. sinensis。(C)2013爱思唯尔有限公司保留所有权利。
C-type lectins (CTLs) have crucial functions in recognizing and eliminating pathogens in innate immunity. This study identified a novel low-density lipoprotein receptor class A (LDLa) domain-containing CTL, designated as EsCTLDcp, from the Chinese mitten crab Eriocheir sinensis. The EsCTLDcp cDNA is 1258 bp long, with a 975 bp open reading frame that encodes a 324-amino acid protein. EsCTLDcp contains a signal peptide, an LDLa, and a single C-type lectin-like domain. EsCTLDcp was only expressed in the hepatopancreas of normal crabs, and its expression was regulated following crab challenge with pathogen-associated molecular patterns and with bacteria. The recombinant EsCTLDcp agglutinates Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Vibrio parahaemolyticus and Aeromonas hydrophila) in the presence of calcium. rEsCTLDcp also binds to various bacteria including S. aureus, Bacillus thuringiensis, Bacillus subtilis, Escherichia coli, Vibrio natriegens, V. parahaemolyticus, and A. hydrophila. The rEsCTLDcp protein helped the crabs clear the virulent Gram-negative bacterium V. parahaemolyticus in vivo, as well as interacted with VP24, an envelope protein of white spot syndrome virus (WSSV). These data suggest that EsCTLDcp functions as a pattern-recognition receptor involved in the innate immunity of E. sinensis. (C) 2013 Elsevier Ltd. All rights reserved.