Higher order cytoskeletal structures.
Higher order cytoskeletal structures.
复制标题
更高阶的细胞骨架结构。
DOI：
10.1091/mbc.e19-12-0679
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发表时间：
2020
影响因子：
3.3
通讯作者：
Feldman,JessicaL
中科院分区：
文献类型：
--
作者：
Avasthi,Prachee;Feldman,JessicaL
细胞骨架聚合物在细胞组织、形态和动力学中起着关键作用。通常，单个聚合物相互作用或与其他蛋白质相互作用，以构建复杂的机器，这是“高阶细胞骨架结构”的焦点。许多演讲者利用改进的可视化策略，克服了各种模式生物的技术挑战，为更高级的细胞骨架组装体的组织和功能提供了新的见解。微管结构和组织会议开始时，重点介绍了微管结构，包括中心粒和基体，微管组织中心（MTOC）和纤毛。马修河汉纳福德（Rusan实验室，国家心肺和血液研究所）发现驱动蛋白-1和动力蛋白在果蝇细胞中中心粒运动沿着非中心体微管中的作用。这些运动需要Pericentrin样蛋白，它作为一个适配器，连接中心粒直接这些马达蛋白。阿丽亚娜·D继续研究非中心体微管的主题。Sanchez（J. Feldman实验室，斯坦福大学）在秀丽隐杆线虫分化的上皮细胞中使用TurboID（一种遗传编码的邻近标记方法）来鉴定非中心体MTOC的关键组分，包括VAB-10 B/Spectraplakin和WDR-62/WDR 62。
Cytoskeletal polymers play key roles in cellular organization, morphology, and dynamics. Often individual polymers interact with each other or additional proteins to build complex machines, which were the focus of the “Higher Order Cytoskeletal Structures” Minisymposium. Many of the speakers in this session utilized improved visualization strategies and overcame technological challenges in a variety of model organisms to provide new insights into the organization and function of higher order cytoskeletal assemblies.Microtubule structures and organization The session opened with talks highlighting microtubule-based structures, including centrioles and basal bodies, microtubule-organizing centers (MTOCs), and cilia. Matthew R. Hannaford (Rusan laboratory, National Heart, Lung and Blood Institute) found a role for kinesin-1 and dynein in centriole motility along noncentrosomal microtubules in Drosophila cells. These movements require Pericentrin-like protein, which serves as an adapter that links centrioles directly to these motor proteins. The theme of noncentrosomal microtubules continued as Ariana D. Sanchez (J. Feldman laboratory, Stanford University) used TurboID, a genetically encoded proximity labeling approach, in Caenorhabditis elegans differentiated epithelial cells to identify key components of noncentrosomal MTOCs including VAB-10B/Spectraplakin and WDR-62/WDR62.