Antibody fusions with fluorescent proteins: a versatile reagent for profiling protein expression
Antibody fusions with fluorescent proteins: a versatile reagent for profiling protein expression
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DOI：
10.1016/s0022-1759(01)00462-8
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发表时间：
2001-11-01
影响因子：
2.2
通讯作者：
Kurosawa, Y
中科院分区：
文献类型：
--
作者：
Morino, K;Katsumi, H;Kurosawa, Y
We developed a system by which antibodies, fused to fluorescent proteins with different wavelengths, can be prepared within a month against various antigens. An antibody library composed of a large number of single-chain Fv-CL fragment was constructed by means of a phage-display system. The constructs were designed to facilitate changing of the protein forms by simple enzyme manipulation. In the present study, we adopted a molecular form of antibody in which a single-chain Fv-CL fragment is fused with a green fluorescent protein (GFP) or red fluorescent protein (RFP). In addition, a His-tag was inserted between CL and GFP (or RFP). We describe the utility of this system using Caenorhabditis elegans embryo as a model. (C) 2001 Elsevier Science B.V. All rights reserved.
