Micro-computed tomography (μ-CT) as a potential tool to assess the effect of dynamic coating routes on the formation of biomimetic apatite layers on 3D-plotted biodegradable polymeric scaffolds
Micro-computed tomography (μ-CT) as a potential tool to assess the effect of dynamic coating routes on the formation of biomimetic apatite layers on 3D-plotted biodegradable polymeric scaffolds
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DOI：
10.1007/s10856-006-0683-8
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发表时间：
2007-02-01
影响因子：
3.7
通讯作者：
Reis, R. L.
中科院分区：
文献类型：
--
作者：
Oliveira, A. L.;Malafaya, P. B.;Reis, R. L.
这项工作研究了动态仿生涂层程序对通过 3D 绘图技术生产的淀粉/聚己内酯 (SPCL) 支架表面骨状磷灰石层生长的影响。这些系统是新提出的用于骨组织工程应用的系统。通过基于硅酸钠的仿生方法生成稳定的磷灰石层后，将支架在静态、搅拌和循环流灌注条件下浸入模拟体液溶液（SBF）中不同的时间段。除了典型的表征技术之外，微计算机断层扫描分析 (mu -CT) 还用于评估支架孔隙率，并作为绘制磷灰石含量的新工具。使用 CT 重建专用软件进行 2D 组织形态分析并创建 3D 虚拟模型。通过所提出的仿生路线，产生了覆盖支架内部的磷灰石层，而不影响其整体形态和互连性。与静态条件相比，动态条件允许由于更高的矿化速率而产生更厚的磷灰石层。 mu-CT 分析清楚地表明，流动灌注是在支架内部获得清晰的磷灰石层的最有效条件。与 SEM 一起，该技术是一种有用的补充工具，用于以非破坏性方式评估磷灰石含量。
This work studies the influence of dynamic biomimetic coating procedures on the growth of bone-like apatite layers at the surface of starch/polycaprolactone (SPCL) scaffolds produced by a 3D-plotting technology. These systems are newly proposed for bone Tissue Engineering applications. After generating stable apatite layers through a sodium silicate-based biomimetic methodology the scaffolds were immersed in Simulated Body Fluid solutions (SBF) under static, agitation and circulating flow perfusion conditions, for different time periods. Besides the typical characterization techniques, Micro-Computed Tomography analysis (mu -CT) was used to assess scaffold porosity and as a new tool for mapping apatite content. 2D histomorphometric analysis was performed and 3D virtual models were created using specific softwares for CT reconstruction. By the proposed biomimetic routes apatite layers were produced covering the interior of the scaffolds, without compromising their overall morphology and interconnectivity. Dynamic conditions allowed for the production of thicker apatite layers as consequence of higher mineralizing rates, when comparing with static conditions. mu -CT analysis clearly demonstrated that flow perfusion was the most effective condition in order to obtain well-defined apatite layers in the inner parts of the scaffolds. Together with SEM, this technique was a useful complementary tool for assessing the apatite content in a non-destructive way.