Mesothelin promotes cell proliferation in the remodeling of neonatal rat pancreas.
Mesothelin promotes cell proliferation in the remodeling of neonatal rat pancreas.
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DOI：
10.3748/wjg.v20.i22.6884
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发表时间：
2014-06
影响因子：
4.3
通讯作者：
D. Yin;Liang-hui You;Q. Yuan;Xiaodi Liang;Ning Wang;Lin-tao Wang;Li Yuan;Ke-ming Wang;W. De
中科院分区：
文献类型：
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作者：
D. Yin;Liang-hui You;Q. Yuan;Xiaodi Liang;Ning Wang;Lin-tao Wang;Li Yuan;Ke-ming Wang;W. De
目的探讨间皮素在新生大鼠内分泌胰腺重构中的作用。方法采用体外细胞培养方法，通过过表达或下调间皮素在INS-1细胞中的表达，观察间皮素对INS-1细胞增殖和凋亡的影响。采用腺病毒介导的RNA干扰技术在体内阻断间皮素的表达，以直接评价间皮素在新生大鼠内分泌胰腺重塑中的作用。结果外源性过表达间皮素可促进INS-1细胞增殖、细胞集落形成及细胞抗凋亡能力。间皮素表达下调对细胞增殖和凋亡无明显影响。与Ad-EGFP组相比，Ad-mesothelin组第7天和第14天胰岛细胞数量明显增多，PCNA表达明显减少。结论间皮素可促进新生大鼠骨重塑期β细胞增殖。间皮素可能在新生大鼠内分泌胰腺重塑中起重要作用。
AIM To investigate the effect of mesothelin in the remodeling of the endocrine pancreas in neonatal rats. METHODS Overexpression or downregulation of mesothelin expression in INS-1 cells was carried out to investigate the effect of mesothelin during cell proliferation and cell apoptosis in vitro. Adenovirus-mediated RNA interference was performed to block mesothelin in vivo to directly assess the role of mesothelin in the remodeling of the endocrine pancreas in neonatal rats. RESULTS Exogenous overexpression of mesothelin promoted cell proliferation, cell colony formation and enhanced cell resistance to apoptosis of INS-1 cells. Down-regulation of mesothelin made no difference in cell proliferation and apoptosis compared with that in the control group. After an injection of adenovirus-mesothelin, a significantly increased number of small islets appeared, and the expression of PCNA was decreased on day 7 and day 14 compared with the Ad-EGFP group. CONCLUSION Mesothelin was able to promote β cell proliferation in the remodeling stage of neonatal rats. Mesothelin may have an important role in the remodeling of the endocrine pancreas in neonatal rats.