High-salt diet promotes crystal deposition through hypertension in Dahl salt-sensitive rat model
High-salt diet promotes crystal deposition through hypertension in Dahl salt-sensitive rat model
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DOI：
10.1111/iju.14035
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发表时间：
2019-06-30
影响因子：
2.6
通讯作者：
Miyazawa, Katsuhito
中科院分区：
文献类型：
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作者：
Nakazawa, Yusuke;Inoue, Shinya;Miyazawa, Katsuhito
目的利用盐敏感性高血压大鼠模型，研究盐诱导高血压对晶体沉积和尿石形成的促进作用。方法用乙二醇和阿法骨化醇诱导雄性Dahl盐敏感大鼠高尿、高钙尿。高血压是由高盐饮食引起的。给予依普利酮，一种选择性盐皮质激素受体拮抗剂。收集血和尿，以评估肾功能，电解质和血肾素-血管紧张素-醛固酮系统。还评价了肾钙含量。组织学检查、基因芯片转录组分析和半定量逆转录聚合酶链反应。结果高盐饮食可增加Dahl盐敏感性高血压大鼠的晶体沉积，依普利酮可显著抑制晶体沉积;晶体形成、生长、粘附和细胞损伤与mRNA表达相关，高盐饮食和乙二醇可调节晶体的表达。结论高盐饮食对盐敏感性高血压和尿石症有促进作用。这种促进作用可以通过依普利酮给药来预防。因此，盐敏感性高血压对Dahl盐敏感大鼠晶体沉积有促进作用。
Objectives To study the promotive effect of salt-induced hypertension on crystal deposition and urolithiasis using a salt-sensitive rat hypertension model. Methods Hyperoxaluria and hypercalciuria were induced in male Dahl salt-sensitive rats with administration of ethylene glycol and alfacalcidol. Hypertension was induced by a high-salt diet. Eplerenone, a selective mineralocorticoid receptor antagonist, was given. Blood and urine were collected to evaluate renal function, electrolytes and the blood renin-angiotensin-aldosterone system. Renal calcium content was also evaluated. Histological examination, transcriptome analysis with DNA microarray and semiquantitative reverse transcriptase polymerase chain reaction were carried out. Results A high-salt diet increased crystal deposition in Dahl salt-sensitive rats with hypertension, and eplerenone administration significantly suppressed it. The mRNA expression profile was associated with crystal formation, growth, adhesion and cellular injury, and it was regulated in the group exposed to a high-salt diet and ethylene glycol. Conclusions A high-salt diet has a promotive effect on salt-sensitive hypertension and urolithiasis. This promotive effect can be prevented by eplerenone administration. Hence, salt-sensitive hypertension has promotive effects on crystal deposition in Dahl salt-sensitive rats.