The involvement of FAK-PI3K-AKT-Rac1 pathway in porcine reproductive and respiratory syndrome virus entry
The involvement of FAK-PI3K-AKT-Rac1 pathway in porcine reproductive and respiratory syndrome virus entry
复制标题
FAK-PI3K-AKT-Rac1通路参与猪繁殖与呼吸综合征病毒侵入
DOI：
10.1016/j.bbrc.2015.01.126
复制
发表时间：
2015-03-06
影响因子：
3.1
通讯作者：
Mao, Xiang
中科院分区：
文献类型：
--
作者：
Ni, Bo;Wen, Li-Bin;Mao, Xiang
CD163和唾液酸粘附素是猪繁殖与呼吸综合征病毒(PRRSV)感染的两种受体。PRRSV进入后激活的信号转导通路报道较少。在我们的研究中，我们证明了PRRSV的进入触发了FAK、PI3K、AKT和rac1的激活。信号通路FAK-PI3K-AKT-rac1是PRRSV进入的关键途径。PF573228阻断FAR可抑制PI3K、ART、rac1的活化和病毒侵入所致的细胞骨架重塑。对FAR、PI3K、AKT和rac1的抑制剂能显著抑制病毒的侵入。综上所述，我们的观察结果表明，PRRSV激活了FAK-PI3K-AKT-rac1信号通路，使其更容易进入细胞。(C)2015 Elsevier Inc.保留所有权利。
CD163 and sialoadhesin had been reported as the two receptors for porcine reproductive and respiratory syndrome virus (PRRSV) infection. The signaling pathway activated by PRRSV entry was seldom reported. In our studies, we demonstrated that PRRSV entry triggers FAK, PI3K, AKT and Rac1 activation. The signaling pathway FAK-PI3K-AKT-Rac1 is essential for PRRSV entry. Blocking FAR by PF573228 attenuates the activation of PI3K, ART, Rac1 and the cytoskeleton remodeling induced by virus entry. Inhibitors to FAR, PI3K, AKT and Rac1 can significantly inhibit the virus entry. In conclusion, our observations reveal that PRRSV triggers the activation of FAK-PI3K-AKT-Rac1 signaling pathway to facilitate its entry into cells. (C) 2015 Elsevier Inc. All rights reserved.