Hepatic properties and transplantation of Thy-1+ cells isolated from ED14 rat fetal liver
Hepatic properties and transplantation of Thy-1+ cells isolated from ED14 rat fetal liver
复制标题
ED14大鼠胎肝Thy-1细胞的肝特性及移植
DOI：
10.1055/s-2007-967854
复制
发表时间：
2007
影响因子：
1.3
通讯作者：
D. Shafritz
中科院分区：
文献类型：
--
作者：
M. Oertel;Anuradha Menthena;Yq Chen;D. Shafritz
Thy-1是造血祖细胞的表型标志物，也在成年大鼠肝脏的活化卵圆细胞中表达。Thy 1+细胞也已在大鼠胎肝中观察到，这些细胞在体外表现出双能肝上皮祖细胞的特性。然而，Thy-1+胎肝细胞的干细胞样特性尚未完全建立，并且没有关于Thy 1+胎肝细胞的肝脏再增殖能力的信息。因此，我们从ED 14胎肝中分离Thy-1+细胞，并检测其体外肝脏基因表达谱和特性，以及移植到成年大鼠肝脏后的增殖和分化潜力，并与Thy-1-胎肝细胞进行比较。在未分级细胞中，Thy-1+为87.2%。在Thy-1+细胞的多个制备物中，仅罕见细胞为AFP阳性（0.1%）;然而，在Thy-1-细胞组分中检测到4.3%的AFP阳性肝母细胞。白蛋白表达分析显示了相似的结果，即Thy 1-与Thy-1+细胞中的白蛋白表达显著更高。此外，CK-19分析显示0.8%的Thy-1+细胞呈阳性。在Thy-1+细胞培养物中，大多数贴壁细胞分散并表现出成纤维细胞样形态。少数细胞形成小的细胞簇，并表现出典型的上皮细胞表达AFP和白蛋白的特征。
Thy–1, a phenotypic marker of hematopoietic progenitor cells, is also expressed in activated oval cells of the adult rat liver. Thy1+ cells have also been observed in the rat fetal liver and these cells exhibit properties of bipotent hepatic epithelial progenitor cells in vitro. However, the stem-like properties of Thy–1+ fetal liver cells have not yet been fully established and there is no information available concerning the liver repopulation capacity of Thy1+ fetal liver cells. We therefore isolated Thy–1+ cells from ED14 fetal liver and examined their hepatic gene expression profile and characteristics in vitro and proliferative and differentiation potential after transplantation into the adult rat liver in comparison to Thy–1-fetal liver cells.Fetal liver cells were selected for Thy–1 expression using immunomagnetic microbeads (MACS) and were enriched from 5.2% Thy–1+ in unfractionated cells to 87.2% Thy–1+. In multiple preparations of Thy–1+ cells, only rare cells were positive for AFP (0.1%); however, 4.3% AFP positive hepatoblasts were detected in Thy–1-cell fractions. Analysis for albumin expression showed similar results, ie, substantially greater albumin expression in Thy1-vs. Thy–1+ cells. In addition, analysis for CK–19 showed 0.8% of the Thy–1+ cells to be positive. In Thy–1+ cell cultures, the majority of attached cells were scattered and exhibited a fibroblastoid morphology. A minority of the cells formed small cell clusters and exhibited typical characteristics of epithelial cells expressing AFP and albumin.