Holliday junction-recognizing protein promotes cell proliferation and correlates with unfavorable clinical outcome of hepatocellular carcinoma.
Holliday junction-recognizing protein promotes cell proliferation and correlates with unfavorable clinical outcome of hepatocellular carcinoma.
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DOI：
10.2147/ott.s127738
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发表时间：
2017
影响因子：
4
通讯作者：
Han M
中科院分区：
文献类型：
--
作者：
Hu B;Wang Q;Wang Y;Chen J;Li P;Han M
目的探讨Holliday连接识别蛋白（HJURP）在肝细胞癌（HCC）中的表达及临床意义。在本研究中，我们检测了164例HCC患者样本中HJURP蛋白的表达，并以此为基础将患者分为HJURP高表达和HJURP低表达两组。我们采用卡方检验分析HJURP表达与临床病理因素的相关性。采用Kaplan-Meier法和log-rank检验确定HJURP的生存意义，采用Cox回归模型确定独立预后因素。通过肝癌细胞系功能测定，我们研究了HJURP对肝癌细胞增殖的影响。在我们的研究中，HJURP高表达的患者比例为25.6%，与肿瘤大小和巴塞罗那临床肝癌分期显著相关。单因素分析证实，HJURP高表达与较差的总生存率（P=0.003）、肿瘤数量（P=0.016）、肿瘤分化（P=0.047）、TNM分期（P=0.005）、巴塞罗那临床肝癌分期（P=0.004）显著相关。多因素分析证实HJURP高表达（P<0.001）是预后不良的独立预后危险因素。实时聚合酶链反应分析显示，HCC组织中HJURP的表达明显高于相应的正常肝组织。此外，我们证明HJURP过表达可以加速HCC细胞系的增殖，而HJURP敲低可以减弱增殖。HJURP高表达是HCC的独立预后生物标志物，预示预后较差。HJURP在HCC细胞增殖中也起重要作用。
To investigate the expression and clinical significance of Holliday junction–recognizing protein (HJURP) in hepatocellular carcinoma (HCC). In this study, we detected the expression of HJURP protein in samples of 164 patients with HCC, and based on this, we divided the patients into two cohorts: high expression of HJURP and low expression of HJURP. We analyzed the correlation between HJURP expression and the clinicopathological factors using chi-square test. Survival significance of HJURP was defined by Kaplan–Meier method and log-rank test, and the independent prognostic factors were identified by Cox regression model. Using function assays of HCC cell lines, we investigated the influence of HJURP on the proliferation of HCC cells. In our study, the proportion of patients with high HJURP expression was 25.6%, which was significantly associated with the tumor size and Barcelona clinic liver cancer stage. Univariate analysis confirmed that high HJURP expression was remarkably associated with poorer overall survival rates (P=0.003), as well as tumor number (P=0.016), tumor differentiation (P=0.047), TNM stage (P=0.005), and Barcelona clinic liver cancer stage (P=0.004). Multivariate analysis confirmed that high HJURP expression (P<0.001) acted as an independent prognostic risk factor of unfavorable prognosis. Real-time polymerase chain reaction analysis revealed that the expression of HJURP was significantly higher in HCC tissues than that in the corresponding normal liver tissues. Moreover, we demonstrated that HJURP overexpression could accelerate HCC cell line proliferation, whereas HJURP knockdown could attenuate the proliferation. High HJURP expression was an independent prognostic biomarker of HCC, predicting poorer prognosis. HJURP also played an important role in HCC cell proliferation.