Selenium Nanoparticles Act Potentially on the Growth Performance, Hemato-Biochemical Indices, Antioxidative, and Immune-Related Genes of European Seabass (Dicentrarchus labrax)
Selenium Nanoparticles Act Potentially on the Growth Performance, Hemato-Biochemical Indices, Antioxidative, and Immune-Related Genes of European Seabass (Dicentrarchus labrax)
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DOI：
10.1007/s12011-020-02431-1
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发表时间：
2020-10-15
影响因子：
3.9
通讯作者：
Dawood, Mahmoud A. O.
中科院分区：
文献类型：
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作者：
Abd El-Kader, Marwa F.;Fath El-Bab, Ahmed F.;Dawood, Mahmoud A. O.
目前的研究调查了硒 (Se) 纳米粒子对欧洲鲈鱼 (Dicentrarchus labrax) 生长性能、血液生化指标、抗氧化和免疫相关基因的作用。因此，初始体重为 20.53 +/- 0.10 g/鱼的鱼饲喂含有 0、0.25、0.5 和 1 mg Se 纳米粒子/kg 饲料的饲料，持续 90 天。饲喂不同水平纳米硒的鱼的最终体重、增重和特定生长率均显着高于对照组，其中饲喂0.5毫克/千克纳米硒组的鱼表现最高，FCR最低。饲喂不同水平硒纳米颗粒的鱼的 Hb、PCV、RBC 和 WBC 值显着高于饲喂基础饲料的鱼。饲喂不同水平硒纳米颗粒的鱼的血清总蛋白和球蛋白值显着高于饲喂基础饲料的鱼。此外，饲喂 0.25 和 0.5 mg 纳米硒/kg 的鱼组的球蛋白值高于饲喂 1 mg 纳米硒/kg 的鱼（P < 0.05）。白蛋白、ALT 和 AST 变量未观察到显着变化 (P > 0.05)。饲喂不同浓度纳米硒的鱼的吞噬指数、吞噬细胞、溶菌酶活性显着高于饲喂基础日粮的鱼，且呈剂量依赖性（P < 0.05）。此外，饲喂 0.25 和 0.5 mg 纳米硒/kg 的鱼组中的 SOD 活性值高于饲喂 1 mg 纳米硒/kg 的鱼组，而饲喂 0.5 mg 纳米硒/kg 饲料的鱼组中的 CAT 活性有所增加（P < 0.05）。膳食纳米硒显着降低了MDA水平，其中喂食0.25毫克/公斤的鱼组的MDA水平最低，其次是喂食0.5和1毫克/公斤的鱼。 GH、IGF-1、IL-8和IL-1β基因的表达在0.25和0.5 mg/kg组中的mRNA水平最高，其次是1 mg/kg组，而HSP70则下调。根据总体结果，建议在日粮中添加纳米硒0.5-1 mg/kg，以维持欧洲鲈鱼的最佳生长性能、血液生化指标、抗氧化状态和免疫相关基因。
The current study investigated the role of selenium (Se) nanoparticles on the growth performance, hemato-biochemical indices, antioxidative, and immune-related genes of European seabass (Dicentrarchus labrax). Therefore, fish with initial weight of 20.53 +/- 0.10 g/fish were fed diets with 0, 0.25, 0.5, and 1 mg Se nanoparticles/kg diet for 90 days. The final body weight, weight gain, and specific growth rate of fish fed dietary nano-Se varying levels were significantly higher than the control with the highest performances and lowest FCR in the group of fish fed nano-Se at 0.5 mg/kg. The values of Hb, PCV, RBCs, and WBCs were significantly higher in fish fed varying levels of Se nanoparticles than fish fed the basal diets. The values of total serum protein and globulin were significantly higher in fish fed varying levels of Se nanoparticles than fish fed the basal diets. Additionally, globulin had higher value in the group of fish fed 0.25 and 0.5 mg nano-Se/kg than fish fed 1 mg nano-Se/kg (P < 0.05). No significant alterations were observed on albumin, ALT, and AST variables (P > 0.05). Phagocytic index, phagocytic, lysozyme activities were significantly higher in fish fed varying levels of Se nanoparticles than fish fed the basal diets in a dose dependent manner (P < 0.05). Further, SOD activity had higher value in the group of fish fed 0.25 and 0.5 mg nano-Se/kg than fish fed 1 mg nano-Se/kg, whereas CAT was increased in the group of fish fed dietary 0.5 mg nano-Se/kg diet (P < 0.05). The level of MDA was significantly lowered by dietary nano-Se where the group of fish fed 0.25 mg/kg had the lowest level followed by those fed 0.5 and 1 mg/kg. The expression ofGH,IGF-1,IL-8, andIL-1 beta genes had the highest mRNA levels in the group of fish fed 0.25 and 0.5 mg/kg followed by those fed 1 mg/kg, whereasHSP70was downregulated. Based on the overall results, Se nanoparticles are recommended at the rate of 0.5-1 mg/kg diet to maintain the optimal growth performance, hemato-biochemical indices, antioxidative status, and immune-related genes in European seabass.