COMPARISON OF GENOMIC DNAS OF DIFFERENT ENTEROCOCCAL ISOLATES USING RESTRICTION ENDONUCLEASES WITH INFREQUENT RECOGNITION SITES
COMPARISON OF GENOMIC DNAS OF DIFFERENT ENTEROCOCCAL ISOLATES USING RESTRICTION ENDONUCLEASES WITH INFREQUENT RECOGNITION SITES
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DOI：
10.1128/jcm.28.9.2059-2063.1990
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发表时间：
1990-09-01
影响因子：
9.4
通讯作者：
WEINSTOCK, GM
中科院分区：
文献类型：
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作者：
MURRAY, BE;SINGH, KV;WEINSTOCK, GM
由于缺乏一种简单有效的菌株比较方法，肠球菌的流行病学评价受到限制。在这项研究中，我们比较了27株分离的粪肠球菌的染色体限制性内切酶酶切模式，从三个不同的位置，使用脉冲场电泳的大染色体片段（14至1000千碱基）。除了两株菌株外，其余的菌株都通过一次裂解和消化产生了清晰的、可评估的图案，其余两株菌株在使用大量细菌时可见。来自不同地点的所有分离株产生不同的限制模式，同一地点的大多数分离株也是如此；也有证据表明菌株在每个地点的个体之间传播。该分析易于进行，并且在模式中观察到的清晰度和多态性表明，该技术将对医院内肠球菌感染的流行病学评估非常有用。
Epidemiologic evaluation of enterococci has been limited by the lack of a simple and effective method for comparing strains. In this study, we have compared chromosomal restriction endonuclease digestion patterns of 27 isolates of Enterococcus faecalis from three different locations by using pulsed-field electrophoresis of large chromosomal fragments (14 to 1,000 kilobases). All but two isolates generated a clear, evaluable pattern with a single lysis and digestion, and the remaining two were visualized when a larger quantity of bacteria was used. All isolates from different locations generated different restriction patterns, as did most isolates within a single location; there was also evidence of spread of strains between individuals in each location. The ease with which this analysis can be performed, together with the clarity and polymorphism seen in the patterns, suggests that this technique will be very useful for epidemiological evaluations of nosocomial enterococcal infections.