LIMB DEVELOPMENT IN THE ABSENCE OF ECTODERMAL RIDGE
LIMB DEVELOPMENT IN THE ABSENCE OF ECTODERMAL RIDGE
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DOI：
10.1038/1841736a0
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发表时间：
1959-01-01
期刊：
影响因子：
64.8
通讯作者：
ZWILLING, E
中科院分区：
文献类型：
--
作者：
BELL, E;SAUNDERS, JW;ZWILLING, E
本通讯旨在表明有关外胚层在鸡胚胎肢体形态发生中的重要性的研究现状。先前的研究1,2,3表明，顶端外胚层脊是肢体生长的诱导因子，当它被移除时，远端结构的发育就停止了。为顶端外胚层脊的重要性提供证据的实验是基于外科手术程序和涉及使用螯合化合物制备无外胚层和胰蛋白酶制备无中胚层的技术。最近，bell4假设外胚层对于肢体的发育可能不是必需的，Amprino和Camosso5也持有同样的观点。在贝尔的实验中，19%的肢体芽通过聚焦超声剥离外胚层并移植到侧翼或cœlom形成了代表肢体所有近端和远端水平的骨骼部分。
THIS communication is designed to indicate the current status of research concerned with the importance of ectoderm in limb morphogenesis in the chick embryo. Previous studies1,2,3indicate that the apical ectodermal ridge serves as an inductor of limb outgrowth, and that elaboration of distal structures ceases when it is removed. The experiments which provide the evidence for the importance of the apical ectodermal ridge are based on surgical procedures1and on techniques which involve the use of a chelating compound for preparing ectoderm-free mesoderm and trypsin for preparing mesoderm-free ectoderm2. Recently Bell4has postulated that the ectoderm may not be necessary for limb elaboration, a view which has also been taken by Amprino and Camosso5. In Bell's experiments, 19 per cent of limb bud stripped of ectoderm by means of focused ultrasound and grafted to the flank or cœlom formed skeletal parts representative of all proximo-distal levels of the limb.