A Novel Function of dcf1 During the Differentiation of Neural Stem Cells In vitro
A Novel Function of dcf1 During the Differentiation of Neural Stem Cells In vitro
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DOI：
10.1007/s10571-008-9266-1
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发表时间：
2008-03
影响因子：
4
通讯作者：
Lei Wang;Jiao Wang;Yiliu Wu;Jie Wu;S. Pang;Rong Pan;T. Wen
中科院分区：
文献类型：
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作者：
Lei Wang;Jiao Wang;Yiliu Wu;Jie Wu;S. Pang;Rong Pan;T. Wen
神经树突形成的研究具有重要的理论意义和应用价值。然而，调控的分子机制仍不清楚。我们以前描述了一种新的EST，它与树突状细胞因子（DCF 1）具有高度同源性，在未分化和分化的神经干细胞（NSCs）之间差异表达。在这项研究中，我们克隆，表达，并沉默dcf1基因，并提供了深入了解其功能，在调节神经干细胞分化过程中树突的形成。结果表明，dcf 1基因编码42 kD的蛋白，在大肠杆菌和神经干细胞中均能成功表达。为了沉默dcf1基因，我们构建了三种不同的siRNA载体，分别转染C17. 2和原代培养的NSCs，使dcf1mRNA表达下调。免疫荧光或GFP分析表明，dcf1基因的过表达，神经干细胞维持在未分化状态。dcf1基因沉默后，细胞倾向于分化为神经元和星形胶质细胞。
The study of neural dendrite formation is of great significance both in theory and applications. However, the molecular mechanisms of regulation remain unclear. We previously described a novel EST, which has high homology with dentritic cell factors (DCF1), expressed differentially between undifferentiated and differentiated neural stem cells (NSCs). In this study, we cloned, expressed, and silenced the dcf1 gene and offered insight into its function in regulating dendrite formation during the differentiation of NSCs. The results indicated that dcf1 encoded a 42 kD protein and could be successfully expressed both inEscherichia coliand NSCs. In order to silence dcf1 gene, three different kinds of siRNA vectors were constructed and transformed into the NSC line C17.2 and primary NSCs, resulting in down regulation of the dcf1 mRNA. Analysis of immunofluorescence or GFP illuminated that with overexpression of the dcf1 gene, the NSCs were maintained in undifferentiated status. After the dcf1 gene was silenced, cells tended to differentiate into neurons and astrocytes.