Effect of Monochromatic Light on Circadian Rhythm of Clock Genes in Chick Pinealocytes
Effect of Monochromatic Light on Circadian Rhythm of Clock Genes in Chick Pinealocytes
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单色光对鸡松果体细胞时钟基因昼夜节律的影响
DOI：
10.1111/php.12963
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发表时间：
2018-11-01
影响因子：
3.3
通讯作者：
Chen, Yaoxing
中科院分区：
文献类型：
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作者：
Ma, Shuhui;Wang, Zixu;Chen, Yaoxing
鸟类的昼夜节律系统是由分布于松果体、视网膜和视交叉上核的相互耦合的起搏器组成的复合体。本研究通过培养鸡原代松果体细胞，分别暴露于红光（RL）、绿色光（GL）、蓝光（BL）、白色光（WL）和恒定黑暗（DD），探讨松果体昼夜节律的自我调节机制。所有照明都设置为12亮：12暗的光周期。7个核心时钟基因（cBmal 1/2、cClock、cCry 1/2和cPer 2/3）、cAanat和褪黑素的24 h表达谱在所有组中均显示出明显的昼夜振荡，但BL中cCry 1节律丧失。与WL相比，GL使正性成分（cClock和cBmal 1/2）的振幅和介导值增大，负性成分（cCry 1/2和cPer 2/3）的振幅和介导值减小。cAanatmRNA和褪黑素分泌的时间模式一直与阳性基因一致。GL使正性元素cAanat和melatonin的顶相提前，RL和BL则相反。因此，GL可能通过增强正时钟基因的表达和抑制负时钟基因的表达来促进褪黑素的分泌。
The avian circadian system is a complex of mutually coupled pacemakers residing in pineal gland, retina and suprachiasmatic nucleus. In this study, the self-regulation mechanism of pineal circadian rhythm was investigated by culturing chick primary pinealocytes exposed to red light (RL), green light (GL), blue light (BL), white light (WL) and constant darkness (DD), respectively. All illuminations were set up with a photoperiod of 12 light: 12 dark. The 24-h expression profiles of seven core clock genes (cBmal1/2, cClock, cCry1/2 and cPer2/3), cAanat and melatonin showed significant circadian oscillation in all groups, except for the loss of cCry1 rhythm in BL. Compared to WL, GL increased the amplitudes and mesors of positive elements (cClock and cBmal1/2) and reduced those of negative elements (cCry1/2 and cPer2/3), in contrast to RL. The temporal patterns of cAanatmRNA and melatonin secretion have always been consistent with the positive genes. Besides, GL advanced the acrophases of the positive elements, cAanat and melatonin, but RL and BL showed the opposite effect. Thereby, GL could promote the secretion of melatonin by enhancing the expressions of positive clock genes and repressing the expressions of negative clock genes.