Regulation of Rad51 function by phosphorylation.
Regulation of Rad51 function by phosphorylation.
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DOI：
10.1038/embor.2011.127
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发表时间：
2011-07-08
期刊：
影响因子：
7.7
通讯作者：
Jackson, Stephen P.
中科院分区：
文献类型：
--
作者：
Flott, Sonja;Kwon, Youngho;Pigli, Ying Zhang;Rice, Phoebe A.;Sung, Patrick;Jackson, Stephen P.
Rad51 is a key enzyme involved in DNA double-strand break repair by homologous recombination. Here, we show that in response to DNA damage, budding yeast Rad51 is phosphorylated on Ser-192 in a manner that is primarily mediated by the DNA-damage responsive protein kinase Mec1. We show that mutating Rad51 Ser-192 to Ala or Glu confers hypersensitivity to DNA damage and homologous recombination defects. Furthermore, biochemical analyses indicate that Ser-192 is required for Rad51 ATP hydrolysis and DNA binding activity in vitro, while mutation of Ser-192 does not markedly interfere with Rad51 multimer formation. These data suggest a model in which Mec1-mediated phosphorylation of Rad51 Ser-192 in response to DNA damage controls Rad51 activity and DNA repair by homologous recombination.
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影响因子：
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影响因子：
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作者：
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