Patterns of synaptic contact upon individually labeled large cells of the dorsal lateral geniculate nucleus of the cat.
Patterns of synaptic contact upon individually labeled large cells of the dorsal lateral geniculate nucleus of the cat.
复制标题
猫背外侧膝状核单独标记的大细胞上的突触接触模式。
DOI：
10.1016/0306-4522(84)90026-5
复制
发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Rosner,MC
中科院分区：
文献类型：
--
作者：
Mason,CA;Guillery,RW;Rosner,MC
对4个密集充满辣根过氧化物酶的大膝状细胞进行了详细的研究，首先在光镜下确定树突的形状和分布，然后在电子显微镜下确定其表面突触接触的模式。其中三个细胞具有1类细胞3的形态特征，1个细胞介于1类和2类细胞之间。所有这些细胞都很少有葡萄样的特征附属物，被认为接受突触小球内的视网膜输入。视网膜传入纤维主要分布在近侧近端树突段和树突分支点附近。一些视网膜传入纤维形成简单的轴突接触，而另一些则形成突触小球的一部分。在核周附近形成突触的视网膜传入纤维均未参与肾小球。在涉及1类细胞的肾小球内，视网膜终末既可以与树突节段相关，特别是在分支点附近，也可以与树突附件相关。少数单一放置的葡萄状附件总是与视网膜传入联系，但不一定是在肾小球排列中。皮质膝状终末多见于周围树突段，而含有多形性小泡(F)的终末大致均匀分布于树突和核周面的各个部位。核周细胞与F型终末相接触，但不与视网膜或皮质膝状终末相接触。一类细长的轴突终末含有圆形突触小泡和很少或没有线粒体，与四个细胞膜中的两个相连，但这些细长轴突的来源尚不清楚。结论：膝状1类细胞的表面可以根据它们获得的突触接触而划分为几个功能截然不同的区域。
Four large geniculate cells that were densely filled with horseradish peroxidase have been studied in detail, at first light microscopically, to define the shape and distribution of the dendritic arbors, and then electron microscopically, to define the pattern of synaptic contacts upon their surfaces. Three of the cells had the morphological characteristics of a class 1 cell3, and one cell was intermediate between class 1 and 2. All of these cells had few of the characteristic grape-like appendages, thought to receive retinal input within synaptic glomeruli. Retinal afferents were mainly distributed around proximal juxtasomatic dendritic segments and dendritic branch points. Some retinal afferents made simple axodendritic contacts, while others formed a part of a synaptic glomerulus. None of the retinal afferents forming synapses near the perikaryon were involved in glomeruli. Within the glomeruli involving class 1 cells, retinal terminals can relate both to dendritic segments, particularly near branch points, as well as to dendritic appendages. The few singly placed grape-like appendages were always contacted by retinal afferents, but not necessarily in a glomerular arrangement. Terminals interpreted as cortico-geniculate were seen most commonly upon peripheral dendritic segments, while those containing pleomorphic vesicles (F) were distributed more or less evenly over all parts of the dendritic and perikaryal surface. The perikaryon itself was contacted by F-type terminals but was not contacted by retino-geniculate or cortico-geniculate terminals. A class of slender axonal terminal, containing round synaptic vesicles and few or no mitochondria, was found contacting two of the four perikarya, but the origin of these slender axons is unknown.It is concluded that the surfaces of geniculate class 1 cells can be separated into several functionally distinct zones on the basis of the synaptic contacts they receive.