Volatile anesthetics inhibit voltage-dependent Ca2+ channels in porcine tracheal smooth muscle cells.
Volatile anesthetics inhibit voltage-dependent Ca2+ channels in porcine tracheal smooth muscle cells.
复制标题
挥发性麻醉剂抑制猪气管平滑肌细胞中电压依赖性 Ca2+ 通道。
DOI：
10.1152/ajplung.1995.268.2.l187
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Croxton,TL
中科院分区：
文献类型：
--
作者：
Yamakage,M;Hirshman,CA;Croxton,TL
吸入麻醉药对气道平滑肌的舒张作用与细胞内游离Ca ~（2+）浓度的降低有关。我们推测，抑制细胞外Ca 2+的进入有助于松弛。因此，我们研究了氟烷，异氟烷，七氟烷对宏观电压激活的钙电流（伊卡）在猪气管平滑肌细胞的影响，使用全细胞膜片钳技术。所有三种挥发性麻醉药均以剂量依赖性方式显著抑制伊卡，诱导的伊卡的电压依赖性无明显变化。对伊卡的抑制作用大小顺序为氟烷<异氟烷<七氟烷。当将数据绘制为脂质相中估计麻醉剂浓度的函数时，三种麻醉剂抑制伊卡的效力无法区分。我们的结论是，挥发性麻醉药在临床相关浓度下对猪气管平滑肌细胞的伊卡具有抑制作用，并且挥发性麻醉药对伊卡的抑制效力与其脂相溶解度密切相关。
The relaxation of airway smooth muscle by volatile anesthetics is associated with a decreased concentration of intracellular free Ca2+. We hypothesized that inhibition of the entry of extracellular Ca2+ contributes to the relaxation. We therefore examined the effects of halothane, isoflurane, and sevoflurane on macroscopic voltage-activated Ca2+ currents (ICa) in porcine tracheal smooth muscle cells, using the whole cell patch-clamp technique. All three volatile anesthetics significantly inhibited ICa in a dose-dependent manner with no apparent shift in the voltage dependence of induced ICa. The order of inhibitory potencies for ICa was halothane < isoflurane < sevoflurane. When data were plotted as a function of the estimated anesthetic concentrations in the lipid phase, the potencies for inhibition of ICa by the three anesthetics were indistinguishable. We conclude that volatile anesthetics have an inhibitory effect on ICa of porcine tracheal smooth muscle cells at clinically relevant concentrations and that the inhibitory potencies of volatile anesthetics on ICa are closely related to their lipid-phase solubilities.