Comparison of changes in beta-tubulin and NF gene expression in rat DRG neurons under regeneration-permissive and regeneration-prohibitive conditions.
Comparison of changes in beta-tubulin and NF gene expression in rat DRG neurons under regeneration-permissive and regeneration-prohibitive conditions.
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再生允许和禁止再生条件下大鼠 DRG 神经元 β-微管蛋白和 NF 基因表达变化的比较。
DOI：
10.1016/0006-8993(94)91238-6
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发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
Oblinger,MM
中科院分区：
文献类型：
--
作者：
Jiang,YQ;Pickett,J;Oblinger,MM
为探讨防止损伤后轴突再生是否会影响神经元对轴突切断的分子反应，采用北方印迹和原位杂交技术研究了大鼠背根神经节（DRG）细胞中神经丝（NF）蛋白和微管蛋白mRNA水平的变化。成年雄性大鼠持续坐骨神经中段的挤压损伤（再生允许条件）或坐骨神经的切割损伤结合近端神经残端的结扎和远端神经的大段的去除（再生禁止条件）。在损伤后14天，在L4和L5 DRG中检测低（NF-L）和中（NF-M）分子量NF蛋白mRNA以及βII-和β III-微管蛋白mRNA的相对水平。数据显示，在坐骨神经的挤压性轴突切断术或切断/结扎性轴突切断术后，DRG中NF-L和NF-M mRNA水平降低，而βII-和β III-微管蛋白mRNA水平升高，这表明轴突切断引起的这些分子变化与轴突再生过程的最终成功或失败无关。然而，切断/结扎轴突切断术比挤压损伤对mRNA的变化有更明显的影响。这一结果表明，再生轴突的反馈机制在调节受损神经元细胞骨架mRNA变化的程度方面很重要。
To examine the question of whether or not prevention of axonal regrowth after injury affects the molecular responses of neurons to axotomy, Northern blotting and in situ hybridization were used to study changes in the mRNA levels of neurofilament (NF) proteins and tubulins in rat dorsal root ganglion (DRG) cells. Adult male rats sustained either a crush lesion of the mid-sciatic nerve (regeneration-permissive condition) or a cut lesion of the sciatic nerve combined with ligation of the proximal nerve stump and removal of a large segment of the distal nerve (regeneration-prohibitive condition). At 14 days post-injury, the relative levels of the low (NF-L) and middle (NF-M) molecular weight NF protein mRNAs, as well as those of βII- and βIII-tubulin, were examined in the L4 and L5 DRG. The data showed that the levels of NF-L and NF-M mRNAs decreased while βII- and βIII-tubulin mRNA levels increased in the DRG after either crush axotomy or cut/ligation axotomy of the sciatic nerve, suggesting that the elicitation of these molecular changes by axon disconnection is independent of the ultimate success or failure of the axonal regrowth process. However, cut/ligation axotomy had a more pronounced effect than did crush injury on the mRNA changes. This result suggests that feedback mechanisms from regrowing axons are important in regulating the extent of the cytoskeletal mRNA changes in injured neurons.