Flow cytometric discrimination of phytoplankton classes by fluorescence emission and excitation properties.
Flow cytometric discrimination of phytoplankton classes by fluorescence emission and excitation properties.
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DOI：
10.1007/bf00865249
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发表时间：
1991-01-01
影响因子：
2.7
通讯作者：
Balfoort, H.W.
中科院分区：
文献类型：
--
作者：
Hofstraat, J.W.;de Vreeze, M.E.J.;Balfoort, H.W.
浮游植物物种的共济失调歧视的基础上获得的荧光数据，通过多波长激发结合波长选择性检测流式细胞术的演示。这种区分是基于物种之间色素成分的差异，这反映在它们的光谱特征中。分类可以通过利用绝对荧光强度和荧光参数比来完成。后一种方法的优点是，在很大程度上减少了与尺寸有关的影响和仪器波动。光适应确实影响获得的绝对参数和比率参数，但并不妨碍将其分类为主要共济失调组。
The ataxonomic discrimination of phytoplankton species on the basis of fluorescence data obtained by multiwavelength excitation in combination with wavelength selective detection in flow cytometry is demonstrated. The discrimination is based on differences in pigment composition between the species, which are reflected in their spectral characteristics. Classification can be done both by making use of the absolute fluorescence intensities and with fluorescence parameter ratios. The latter approach has the advantage that size-related effects and instrument fluctuations are reduced to a large extent. Photoadaptation does influence the absolute as well as the ratioed parameters that are obtained but does not impede the classification into major ataxonomic groups.