Electropolymerization-Induced Positively Charged Phenothiazine Polymer Photoelectrode for Highly Sensitive Photoelectrochemical Biosensing
Electropolymerization-Induced Positively Charged Phenothiazine Polymer Photoelectrode for Highly Sensitive Photoelectrochemical Biosensing
复制标题
用于高灵敏光电化学生物传感的电聚合诱导带正电吩噻嗪聚合物光电极
DOI：
10.1021/acs.analchem.9b03311
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发表时间：
2019
影响因子：
7.4
通讯作者：
Li Feng
中科院分区：
文献类型：
--
作者：
Wang Jiao;Lv Wenxin;Wu Jiahui;Li Haiyin;Li Feng
探索一种具有高光电转换效率和丰富官能团的电极，用于理想光电化学（PEC）传感器的开发是迫在眉睫的，但也面临着重大挑战。本文报道了一种在氧化铟锡（ITO）表面（PPT/ITO）上制备吩噻嗪聚合物膜的电聚合策略，仅在几秒钟内和单体。所制备的PPT/ITO电极具有良好的稳定性和丰富的季铵盐基团，可以通过与带负电荷的材料静电结合来开发高灵敏度的PEC传感器。在此背景下，提出了一种CdS qds功能化PPT/ITO电极（CdS/PPT/ITO），并将其应用于毒死蜱的分析中，毒死蜱是有机磷农药（OP）的模型靶标。乙酰胆碱酯酶（AChE）诱导乙酰硫代胆碱（ATCh）水解产生的硫代胆碱通过静电斥力使CdS量子点远离PPT/ITO，从而降低PEC电流，而毒死蜱通过抑制AChE活性抑制硫代胆碱的生成。与没有毒死蜱的情况相比，测定了显著增强的PEC电流，并与毒死蜱的量成正比。因此，所开发的基于cd /PPT/ ito的PEC传感器实现了出色的毒死蜱生物传感，灵敏度提高到约ng/mL水平，特异性良好。我们设想该策略将为方便地制作具有高性能的光电极提供新的途径，这将在PEC传感中有更有用的应用。
Exploring the fabrication of an electrode with high photoelectric conversion efficiency and abundant functional groups for ideal photoelectrochemical (PEC) sensor development is highly urgent but faces a significant challenge. Herein we report an electropolymerization strategy for the preparation of phenothiazine polymeric film on an indium tin oxide (ITO) surface (PPT/ITO), within only a few seconds, and monomers. The fabricated PPT/ITO electrode possessed excellent stability and abundant quaternary ammonium salt groups for developing a highly sensitive PEC sensor through electrostatic binding with negatively charged materials. In this context, a CdS QDs-functionalized PPT/ITO electrode (CdS/PPT/ITO) was proposed and applied to the analysis of chlorpyrifos, used as a model target organophosphorous pesticide (OP). The thiocholine generated from acetylcholinesterase (AChE)-induced catalyzed hydrolysis of acetylthiocholine (ATCh) efficiently directed CdS QDs away from PPT/ITO via electrostatic repulsion, subsequently decreasing PEC current, whereas chlorpyrifos prohibited the generation of thiocholine through inhibiting AChE activity. As compared to the case where chlorpyrifos is absent, significantly enhanced PEC current is determined and is proportional to chlorpyrifos amounts. Thus, the developed CdS/PPT/ITO-based PEC sensor achieved excellent chlorpyrifos biosensing with improved sensitivity down to approximately ng/mL level with good specificity. We envision the proposed strategy will provide a new path to conveniently fabricate photoelectrodes possessing high performance, which will have more useful applications in PEC sensing.