DEVELOPMENTAL AND AGE-RELATED PROCESSES THAT INFLUENCE THE LONGEVITY AND SENESCENCE OF PHOTOSYNTHETIC TISSUES IN ARABIDOPOSIS
DEVELOPMENTAL AND AGE-RELATED PROCESSES THAT INFLUENCE THE LONGEVITY AND SENESCENCE OF PHOTOSYNTHETIC TISSUES IN ARABIDOPOSIS
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DOI：
10.1105/tpc.5.5.553
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发表时间：
1993-05-01
期刊：
影响因子：
11.6
通讯作者：
BLEECKER, AB
中科院分区：
文献类型：
--
作者：
HENSEL, LL;GRBIC, V;BLEECKER, AB
研究了影响拟南芥光合组织寿命和衰老的因素。为了确定生殖发育对体细胞组织衰老时间的影响，比较了兰茨贝格直立株和开花延迟（CO-2）或产生不育花（MS 1 -1）的等基因突变株的莲座叶的寿命。在这些品系之间观察到单个叶片衰老的时间没有差异，表明体细胞组织寿命不受该物种生殖发育的影响。为了研究差异基因表达在叶片衰老过程中的作用，分离了代表衰老组织中差异表达基因的cDNA克隆。一个这样的克隆的序列分析表明同源性，以前克隆的半胱氨酸蛋白酶，这是一致的，该基因的产物在氮补救的作用。RNA凝胶印迹分析表明，衰老相关基因的表达增加之前，在光合作用和光合作用相关基因的表达下降。提出了一个模型，其中它被假定，叶片衰老是由年龄相关的光合作用过程中的下降。
Factors that influence the longevity and senescence of photosynthetic tissues of Arabidopsis were investigated. To determine the influence of reproductive development on the timing of somatic tissue senescence, the longevity of rosette leaves of the Landsberg erecta strain and of isogenic mutant lines in which flowering is delayed (co-2) or sterile flowers are produced (ms1-1) were compared. No difference in the timing of senescence of individual leaves was observed between these lines, indicating that somatic tissue longevity is not governed by reproductive development in this species. To examine the role of differential gene expression in the process of leaf senescence, cDNA clones representing genes that are differentially expressed in senescing tissues were isolated. Sequence analysis of one such clone indicated homology to previously cloned cysteine proteinases, which is consistent with a role for the product of this gene in nitrogen salvage. RNA gel blot analysis revealed that increased expression of senescence-associated genes is preceded by declines in photosynthesis and in the expression of photosynthesis-associated genes. A model is presented in which it is postulated that leaf senescence is triggered by age-related declines in photosynthetic processes.