Lacto-ghrestatin, a novel bovine milk-derived peptide, suppresses ghrelin secretion
Lacto-ghrestatin, a novel bovine milk-derived peptide, suppresses ghrelin secretion
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DOI：
10.1002/1873-3468.12731
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发表时间：
2017-07-01
期刊：
影响因子：
3.5
通讯作者：
Ohinata, Kousaku
中科院分区：
文献类型：
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作者：
Aoki, Hayato;Nakato, Junya;Ohinata, Kousaku
Ghrelin是一种从胃中分离的内源性肽，已知其在外周给药后刺激食物摄入。我们发现β-乳球蛋白的酶消化减少了生长素释放肽产生细胞系MGN 3 -1的生长素释放肽分泌。使用nanoLC-OrbitrapMS全面分析消化物中存在的肽。其中，我们鉴定了对应于β-乳球蛋白（1-9）的九肽LIVTQTMKG抑制来自MGN 3 -1细胞的生长素释放肽分泌。我们将LIVTQTMKG命名为“乳糖生长激素”。我们发现，乳糖-生长激素抑制素降低细胞内cAMP水平和生长激素释放肽生产相关基因的mRNA表达水平在MGN 3 -1细胞。口服乳促生长素抑制素可降低禁食小鼠的血浆促生长素释放肽水平和食物摄入量。乳促生长素抑制素是第一个在体外和体内抑制生长素释放肽分泌的食物源性肽。
Ghrelin, an endogenous peptide isolated from the stomach, is known to stimulate food intake after peripheral administration. We found that the enzymatic digest of beta-lactoglobulin decreases ghrelin secretion from the ghrelin-producing cell line MGN3-1. The peptides present in the digest were comprehensively analyzed using the nanoLC-OrbitrapMS. Among them, we identified that the nonapeptide LIVTQTMKG, corresponding to beta-lactoglobulin(1-9), suppresses ghrelin secretion from MGN3-1 cells. We named LIVTQTMKG 'lacto-ghrestatin'. We found that lacto-ghrestatin decreases intracellular cAMP levels and mRNA expression levels of ghrelin production-related genes in MGN3-1 cells. Orally administered lacto-ghrestatin decreases plasma ghrelin levels and food intake in fasted mice. Lacto-ghrestatin is the first food-derived peptide to suppress ghrelin secretion in vitro and in vivo.