Dilution cultivation of marine heterotrophic bacteria abundant after a spring phytoplankton bloom in the North Sea
Dilution cultivation of marine heterotrophic bacteria abundant after a spring phytoplankton bloom in the North Sea
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DOI：
10.1111/1462-2920.12479
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发表时间：
2015-10-01
影响因子：
5.1
通讯作者：
Harder, Jens
中科院分区：
文献类型：
--
作者：
Hahnke, Richard L.;Bennke, Christin M.;Harder, Jens
单个浮游细菌物种在藻类生物量再矿化中的作用知之甚少。来自分子数据的证据表明，2009年北海德国湾春季硅藻大量繁殖之后，迅速出现了未培养的浮游细菌物种，包括石腐杆菌属、福尔摩沙属、偏振杆菌属（黄细菌纲）和Reinekea属（伽马变形菌纲）。我们分离菌株从同一地点在2010年春季硅藻水华使用稀释培养在人工海水培养基微摩尔底物和营养浓度。流式细胞仪显示细胞从单个细胞生长到10（4）-10（6）个细胞/ml的密度和35%的可培养性。2009年春、夏分离到新的福尔摩沙、极化杆菌和Reinekea菌株，其16 S rRNA基因序列与浮游细菌的同源性>99.8%。2009年，对选定分离株的基因组进行了测序草案，并用于浮游细菌宏基因组的读取招募。宏基因组读数覆盖福尔摩沙进化枝B的93%、Reinekea的91%和福尔摩沙进化枝A基因组的74%，应用94.5%核苷酸同一性阈值。这些新菌株代表了丰富的浮游细菌物种在北海沿海浮游植物水华中蓬勃发展。
The roles of individual bacterioplankton species in the re-mineralization of algal biomass are poorly understood. Evidence from molecular data had indicated that a spring diatom bloom in the German Bight of the North Sea in 2009 was followed by a rapid succession of uncultivated bacterioplankton species, including members of the genera Ulvibacter, Formosa, Polaribacter (class Flavobacteria) and Reinekea (class Gammaproteobacteria). We isolated strains from the same site during the diatom bloom in spring 2010 using dilution cultivation in an artificial seawater medium with micromolar substrate and nutrient concentrations. Flow cytometry demonstrated growth from single cells to densities of 10(4)-10(6)cellsml(-1) and a culturability of 35%. Novel Formosa, Polaribacter and Reinekea strains were isolated and had 16S rRNA gene sequence identities of >99.8% with bacterioplankton in spring or summer 2009. Genomes of selected isolates were draft sequenced and used for read recruitment of metagenomes from bacterioplankton in 2009. Metagenome reads covered 93% of a Formosa clade B, 91% of a Reinekea and 74% of a Formosa clade A genome, applying a 94.5% nucleotide identity threshold. These novel strains represent abundant bacterioplankton species thriving on coastal phytoplankton blooms in the North Sea.