A new basophil activation test using CD63 and CCR3 in allergy to antibiotics
A new basophil activation test using CD63 and CCR3 in allergy to antibiotics
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DOI：
10.1111/j.1365-2222.2009.03426.x
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发表时间：
2010-03-01
影响因子：
6.1
通讯作者：
Ring, J.
中科院分区：
文献类型：
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作者：
Eberlein, B.;Suarez, I. Leon;Ring, J.
流式细胞术嗜碱性粒细胞激活试验（BAT）已发展成为体外诊断ige介导反应的细胞试验。在各种过敏原刺激后使用了不同的标记物和技术。目的本研究的目的是比较已建立的BAT （Flow-CAST (R)）和新开发的使用CD63和CCR3 （Flow2 CAST (R)）的嗜碱性粒细胞激活方案在i型抗生素过敏患者中的应用。材料与方法对24例有1型抗生素过敏史的患者进行调查。仔细记录过敏史，并进行皮肤试验和特异性IgE抗体测定。在不同浓度的抗生素刺激后，进行了两种不同的BAT，使用CD63表达但不同的方案。15名无抗生素过敏史的健康受试者作为对照。结果Flow2 CAST (R)对患者病史的敏感性高于Flow-CAST (R)（55%对53%）。Flow2 CAST (R)和Flow-CAST (R)对于阴性病史和阴性RAST的对照组的特异性均为80%。结论两种不同的BAT作为细胞试验对抗生素过敏患者的体外诊断具有相同的特异性，且Flow2 CAST (R)的敏感性略高。引用本文：B. Eberlein， I. Leon Suarez， U. Darsow， F. Rueff， H. Behrendt， J. Ring，临床与实验过敏，2010(40)411-418。
P>BackgroundFlow cytometric basophil activation tests (BAT) have been developed as cellular tests for in vitro diagnosis of IgE-mediated reactions. Different markers and techniques have been used after stimulation with various allergens.ObjectiveIt was the aim of the present study to compare an established BAT (Flow-CAST (R)) with a newly developed basophil activation protocol using CD63 and CCR3 (Flow2 CAST (R)) in patients with type-I allergy to antibiotics.Materials and methodsTwenty-four patients with a history of type-I allergy to antibiotics were examined. A careful allergy history was taken, and skin tests and determination of specific IgE antibodies were performed. Two different BAT using CD63 expression but different protocols were carried out after stimulation with different concentrations of antibiotics. Fifteen healthy subjects without a history of antibiotic allergy were studied as controls.ResultsThe Flow2 CAST (R) showed a higher sensitivity than the Flow-CAST (R) (55% vs. 53%) with regard to patients' history. Specificity was 80% both for the Flow2 CAST (R) and for the Flow-CAST (R) with regard to controls with negative history and negative RAST.ConclusionThese results show the value of two different BAT as cellular tests in the in vitro diagnosis of patients with antibiotic allergy with equal specificity and a slightly higher sensitivity for the Flow2 CAST (R).Cite this as: B. Eberlein, I. Leon Suarez, U. Darsow, F. Rueff, H. Behrendt and J. Ring, Clinical & Experimental Allergy, 2010 (40) 411-418.