Androgen regulation of molecular forms of beta-D-glucuronidase in the mouse epididymis: comparison with liver and kidney.
Androgen regulation of molecular forms of beta-D-glucuronidase in the mouse epididymis: comparison with liver and kidney.
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DOI：
10.1002/j.1939-4640.1996.tb01775.x
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发表时间：
1996-05
影响因子：
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通讯作者：
A. Abou-haila;Daulat R. P. Tulsiani;M. Skudlarek;M. Orgebin‐Crist
中科院分区：
文献类型：
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作者：
A. Abou-haila;Daulat R. P. Tulsiani;M. Skudlarek;M. Orgebin‐Crist
研究了β -葡糖醛酸酶分子形式在小鼠附睾、肝脏和肾脏中的表达和雄激素调控。在非变性条件下进行的二维聚丙烯酰胺凝胶电泳显示，与含有四种微粒体（M1-M4）和一种主要溶酶体(L)形式的β -葡糖醛酸酶的肝脏和肾脏相比，附睾在各种分子形式的酶表达方面显示出区域差异和组织特异性。只有溶酶体形式（pI 5.4-6.1）存在于附睾头，而体/尾则包含溶酶体形式，自由X形式（pI 5.9-6.3）和四种微粒体形式（X形式与egasyn复合）。缺乏egasyn的突变小鼠在附睾远端没有微粒体形式。在附睾液中，溶酶体形式遍及整个附睾，而X型仅出现在附睾体/尾。十二烷基硫酸钠（SDS）凝胶电泳和免疫沉淀β -葡萄糖醛酸酶免疫印迹分析显示，附睾头只有一条对应于L型（表观分子量为74 kDa）的条带，而体/尾有两条对应于L型和X型的条带（表观分子量为73和75 kDa）。小鼠去势抑制了附睾X型和M型形态的区域差异。睾酮补充阉割小鼠恢复附睾头β -葡糖醛酸酶的特征电泳模式。在肝脏和肾脏中，去势对分子形式的表达没有影响，而雄激素处理在肾脏中诱导了X形式。β -葡糖醛酸酶的组织化学定位证实了在附睾中所见的区域特异性，此外还揭示了β -葡糖醛酸酶表达的细胞特异性。这些结果表明-葡萄糖醛酸酶具有组织特异性，在附睾的情况下，具有区域和细胞特异性。此外，不同组织中的酶对雄激素的反应也不同。
The expression and androgen regulation of beta-glucuronidase molecular forms were examined in mouse epididymis, liver, and kidney. Two-dimensional polyacrylamide gel electrophoresis performed under nondenaturing conditions showed that, compared to liver and kidney, which contain four microsomal (M1-M4) and a major lysosomal (L) form of beta-glucuronidase, the epididymis revealed regional differences and tissue specificity in the expression of the various molecular forms of the enzyme. Only the lysosomal form (pI 5.4-6.1) is present in the caput epididymidis while the corpus/cauda contains the lysosomal form, the free X form (pI 5.9-6.3) and the four microsomal forms (X form complexed with egasyn). Mutant mice that lack egasyn have no microsomal forms in the distal epididymis. In epididymal fluid, the lysosomal form is found throughout the epididymis, whereas the X form appears only in the corpus/cauda epididymidis. Sodium dodecyl Sulfate (SDS)-gel electrophoresis and western blot analysis of immunoprecipitated beta-glucuronidase revealed only one band corresponding to the L form (apparent molecular weight 74 kDa) in the caput epididymidis and two bands in the corpus/cauda (apparent molecular weights 73 and 75 kDa), corresponding to L and X forms, respectively. Castration of mice led to the suppression of the regional differences in the appearance of X and M forms in the epididymis. Testosterone supplementation to castrated mice restored the characteristic electrophoretic pattern of beta-glucuronidase in the caput epididymidis. In the liver and kidney, castration has no effect on the expression of the molecular forms, whereas androgen treatment induced the X form in the kidney. Histochemical localization of beta-glucuronidase confirmed the region specificity seen in the epididymis and in addition revealed cell specificity in the expression of beta-glucuronidase. These results indicate that beta-glucuronidase shows tissue specificity and, in the case of the epididymis, region and cell specificity. In addition, the enzyme in the different tissues responds differentially to androgens.