Synapse-specific expression of mu opioid receptor long-term depression in the dorsomedial striatum.
Synapse-specific expression of mu opioid receptor long-term depression in the dorsomedial striatum.
复制标题
背内侧纹状体中μ阿片受体长期抑制的突触特异性表达。
DOI：
10.1038/s41598-020-64203-0
复制
发表时间：
2020
影响因子：
4.6
通讯作者：
Atwood,BradyK
中科院分区：
文献类型：
--
作者：
Muñoz,Braulio;Haggerty,DavidL;Atwood,BradyK
背侧纹状体是参与动作控制的大脑区域，背内侧纹状体（DMS）介导目标导向动作，背外侧纹状体（DLS）介导习惯性动作。突触前长时程突触抑制（LTD）的可塑性在背侧纹状体的突触能输入介导许多背侧纹状体依赖的行为和LTD的中断影响动作控制。我们以前的工作确定了μ阿片受体（MORs）作为突触特异性形式的突触抑制在一些不同的DLS突触的介质。我们证明，前岛叶皮层输入是唯一的输入，表达酒精敏感的MOR介导的LTD（MOP-LTD）在DLS。在这里，我们探索mOP-LTD在DMS使用小鼠脑切片电生理学。我们发现，与DLS相反，DMS mOP-LTD是通过激活前扣带回和内侧前额叶皮质以及基底外侧杏仁核输入和纹状体胆碱能中间神经元突触到DMS中棘神经元的输入处的MOR诱导的，这表明DMS中的莫尔突触可塑性比DLS中的突触特异性低。此外，只有皮层输入的mOP-LTD对酒精的有害作用敏感。这些结果表明，酒精诱导的神经适应性差异表达的突触特异性的方式，并可能在目标导向和习惯性行为的改变中发挥作用。
The dorsal striatum is a brain region involved in action control, with dorsomedial striatum (DMS) mediating goal-directed actions and dorsolateral striatum (DLS) mediating habitual actions. Presynaptic long-term synaptic depression (LTD) plasticity at glutamatergic inputs to dorsal striatum mediates many dorsal striatum-dependent behaviors and disruption of LTD influences action control. Our previous work identified mu opioid receptors (MORs) as mediators of synapse-specific forms of synaptic depression at a number of different DLS synapses. We demonstrated that anterior insular cortex inputs are the sole inputs that express alcohol-sensitive MOR-mediated LTD (mOP-LTD) in DLS. Here, we explore mOP-LTD in DMS using mouse brain slice electrophysiology. We found that contrary to DLS, DMS mOP-LTD is induced by activation of MORs at inputs from both anterior cingulate and medial prefrontal cortices as well as at basolateral amygdala inputs and striatal cholinergic interneuron synapses on to DMS medium spiny neurons, suggesting that MOR synaptic plasticity in DMS is less synapse-specific than in DLS. Furthermore, only mOP-LTD at cortical inputs was sensitive to alcohol’s deleterious effects. These results suggest that alcohol-induced neuroadaptations are differentially expressed in a synapse-specific manner and could be playing a role in alterations of goal-directed and habitual behaviors.