Expression Patterns of Three UGT Genes in Different Chemotype Safflower Lines and under MeJA Stimulus Revealed Their Potential Role in Flavonoid Biosynthesis.
Expression Patterns of Three UGT Genes in Different Chemotype Safflower Lines and under MeJA Stimulus Revealed Their Potential Role in Flavonoid Biosynthesis.
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不同化学型红花品系和 MeJA 刺激下三个 UGT 基因的表达模式揭示了它们在类黄酮生物合成中的潜在作用
DOI：
10.1371/journal.pone.0158159
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Guo ML
中科院分区：
文献类型：
--
作者：
Guo DD;Liu F;Tu YH;He BX;Gao Y;Guo ML
红花（红花）在中国作为药用植物受到了极大的关注。黄酮类化合物是主要的活性药物化合物。UDP-糖基转移酶在红花黄酮类化合物的合成和贮藏过程中起重要作用。在这项研究中，45 UGT unigenes筛选从我们的转录数据库的红花。其中27个UGT单基因具有完整的开放阅读框架，其等电点和分子量各不相同。系统进化树显示，CtUGT 3和CtUGT 16属于UGT 71亚家族，参与代谢过程，而CtUGT 25与PoUGT高度同源，均催化黄酮类化合物的糖基化，属于UGT 90亚家族。基因芯片显示，这三个UGT基因在两个化学型红花品系中表现出时间差异。为了功能性地表征红花中的UGT，克隆并分析了CtUGT 3、CtUGT 16和CtUGT 25。亚细胞定位表明，这三个UGT可能位于细胞质和叶绿体中。表达模式表明，这三个UGT都被抑制在两个线响应茉莉酸甲酯诱导。表达模式和代谢产物积累的共表达关系表明，在黄线中，CtUGT 3和CtUGT 25与山奈酚-3-O-β-D-葡萄糖苷正相关，CtUGT 16与槲皮素-3-O-β-D-葡萄糖苷正相关，而在白色线中，CtUGT 3和CtUGT 25与槲皮素-3-O-β-D-葡萄糖苷正相关。本研究表明，三个CtUGT在两个红花品系中表现出不同的功能特性，在黄酮类化合物的生物合成中发挥着重要的和多重的作用。
Safflower (Carthamus tinctorius L.) has received a significant amount of attention as a medicinal plant in China. Flavonoids are the dominant active medical compounds. UDP-glycosyltransferase plays an essential role in the biosynthesis and storage of flavonoids in safflower. In this study, 45 UGT unigenes were screened from our transcriptomic database of safflower. Among them, 27 UGT unigenes were predicted to own a complete open reading frame with various pI and Mw. The phylogenetic tree showed that CtUGT3 and CtUGT16 were classified under the UGT71 subfamily involved in metabolite process, whereas CtUGT25 has high identities with PoUGT both catalyzing the glycosylation of flavonoids and belonging to the UGT90 subfamily. cDNA microarray exhibited that the three UGT genes displayed temporal difference in two chemotype safflower lines. To functionally characterize UGT in safflower, CtUGT3, CtUGT16 and CtUGT25 were cloned and analyzed. Subcellular localization suggested that the three UGTs might be located in the cell cytoplasm and chloroplast. The expression pattern showed that the three UGTs were all suppressed in two lines responsive to methyl jasmonate induction. The co-expression relation of expression pattern and metabolite accumulation demonstrated that CtUGT3 and CtUGT25 were positively related to kaempferol-3-O-β-D-glucoside and CtUGT16 was positively related to quercetin-3-O-β-D-glucoside in yellow line, whereas CtUGT3 and CtUGT25 were positively related to quercetin-3-O-β-D-glucoside in white line. This study indicates that the three CtUGTs play a significant and multiple role in flavonoids biosynthesis with presenting different functional characterization in two safflower lines.