Development of a bio-electrochemical assay for AFB1 detection in olive oil
Development of a bio-electrochemical assay for AFB1 detection in olive oil
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DOI：
10.1016/j.bios.2008.10.002
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发表时间：
2009-03-15
影响因子：
12.6
通讯作者：
Palleschi, Giuseppe
中科院分区：
文献类型：
--
作者：
Ben Rejeb, Ines;Arduini, Fabiana;Palleschi, Giuseppe
基于黄曲霉毒素B-1（AFB（1））对乙酰胆碱酯酶（AChE）的抑制作用，提出了一种新的黄曲霉毒素生物传感器检测方法。AChE存在于溶液中，并且使用电流型胆碱氧化酶生物传感器来监测其残余活性。为了产生生物传感器，胆碱氧化酶通过交联固定到用普鲁士蓝（PB）修饰的丝网印刷电极上，并且这些电极用于在低电位（相对于丝网印刷内部银伪参比电极为-0.05 V）下检测H2 O2。为了开发AFB（1）测定法，对AChE和底物浓度、甲醇效应和pH等几个参数进行了评价和优化。线性工作范围经评估为10-60 ppb。在预浓缩步骤后，检测到低至2 ppb的浓度，相当于人类食品中黄曲霉毒素B1的法律的限度。使用市售橄榄油对样品进行了方法适用性评价。橄榄油样品中10 ppb黄曲霉毒素B（1）的回收率为78 +/- 9%。(C)2008 Elsevier B. V.保留所有权利。
A novel biosensor assay format for aflatoxin based on acetylcholinesterase (AChE) inhibition by aflatoxin B-1 (AFB(1)) is proposed. The AChE was present in solution and an amperometric choline oxidase biosensor was used for monitoring its residual activity. To create the biosensor, the choline oxidase was immobilized by cross-linking onto screen-printed electrodes modified with Prussian Blue (PB) and these were used to detect the H2O2 at low potential (-0.05 V versus a screen-printed internal silver pseudoreference electrode). For the development of the AFB(1) assay, several parameters such as AChE and substrate concentration, the methanol effect, and pH were evaluated and optimized. The linear working range was assessed to be 10-60 ppb. Concentrations as low as 2 ppb, which correspond to the legal limit of AFB1 in food for humans, were detected after a pre-concentration step. The suitability of the method was evaluated using commercial olive on samples. A recovery equal to 78 +/- 9% for 10 ppb of AFB(1) in olive oil samples was obtained. (C) 2008 Elsevier B.V. All rights reserved.