The antioxidant status, apoptosis, intercellular integrity and immune function of grass carp (Ctenopharyngodon idella) head kidney and spleen fed different levels of pyridoxine
The antioxidant status, apoptosis, intercellular integrity and immune function of grass carp (Ctenopharyngodon idella) head kidney and spleen fed different levels of pyridoxine
复制标题
不同浓度吡哆醇饲喂草鱼头肾、脾脏的抗氧化状态、细胞凋亡、细胞间完整性和免疫功能
DOI：
10.1111/anu.13022
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发表时间：
2019-12-01
影响因子：
3.5
通讯作者：
Zhou, Xiao-Qiu
中科院分区：
文献类型：
--
作者：
Jiang, Wei-Dan;Zheng, Xin;Zhou, Xiao-Qiu
研究了饲料中添加吡哆醇（PN）对草鱼幼鱼头肾和脾脏抗氧化状态、细胞凋亡、细胞间完整性和免疫功能的影响。结果表明，与最佳PN水平相比，PN缺乏降低了抗氧化酶活性，下调了抗氧化酶、NF-E2相关因子2（Nrf 2）、髓细胞白血病-1（Mcl-1）和紧密连接蛋白的mRNA水平，增加了活性氧（ROS）、丙二醛（MDA）和蛋白羰基（PC）的含量，上调了半胱氨酸天冬氨酸蛋白酶（caspase）、Fas配体（FasL）、p38丝裂原活化蛋白激酶（p38 MAPK）和肌球蛋白轻链激酶（MLCK）的mRNA水平（p < .05）。(b)PN缺乏使溶菌酶（LZ）、酸性磷酸酶（ACP）活性和补体含量降低，并使抗菌肽、抗炎细胞因子、κ B α抑制因子的mRNA水平降低（I κ B α）和雷帕霉素的靶点（TOR），而上调促炎细胞因子和核因子κ B p65的mRNA水平在鱼的头肾和脾脏中的NF-κ Bp 65（p <0.05）。(c)根据丙二醛含量和溶菌酶活性估算草鱼的饲料PN需要量分别为4.97和4.99 mg/kg饲料。
This study was carried out to investigate the effects of dietary pyridoxine (PN) on the antioxidant status, apoptosis, intercellular integrity and immune function of head kidney and spleen in young grass carp (Ctenopharyngodon idella). Results showed that compared with the optimal PN level, (a) PN deficiency decreased antioxidant enzyme activities and down-regulated the mRNA levels of antioxidant enzymes, NF-E2-related factor 2 (Nrf2), myeloid cell leukaemia-1 (Mcl-1) and tight junction proteins, whereas it increased the contents of reactive oxygen species (ROS), malondialdehyde (MDA) and protein carbonyl (PC), and up-regulated the mRNA levels of cysteinyl aspartic acid-protease (caspase), Fas ligand (FasL), p38 mitogen-activated protein kinase (p38MAPK) and myosin light chain kinase (MLCK) in the head kidney and spleen of fish (p < .05). (b) PN deficiency decreased lysozyme (LZ) and acid phosphatase (ACP) activities, and complement contents, and down-regulated the mRNA levels of antibacterial peptides, anti-inflammatory cytokines, inhibitor of kappa B alpha (I kappa B alpha) and target of rapamycin (TOR), whereas it up-regulated the mRNA levels of pro-inflammatory cytokines and nuclear factor kappa B p65 (NF-kappa Bp65) in the head kidney and spleen of fish (p < .05). (c) Dietary PN requirements of grass carp based on the malondialdehyde content and lysozyme activity were estimated as 4.97 and 4.99 mg/kg diet, respectively.