Paracellular Pathway-Mediated Mycoplasma hyopneumoniae Migration across Porcine Airway Epithelial Barrier under Air-Liquid Interface Conditions.
Paracellular Pathway-Mediated Mycoplasma hyopneumoniae Migration across Porcine Airway Epithelial Barrier under Air-Liquid Interface Conditions.
复制标题
气液界面条件下细胞旁途径介导的猪肺炎支原体跨猪气道上皮屏障的迁移。
DOI：
10.1128/iai.00470-20
复制
发表时间：
2020
影响因子：
3.1
通讯作者：
Feng Zhixin
中科院分区：
文献类型：
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作者：
Wang Haiyan;Zhang Zhenzhen;Xie Xing;Liu Beibei;Wei Yanna;Gan Yuan;Yuan Ting;Ni Bo;Wang Jia;Zhang Lei;Xiong Qiyan;Shao Guoqing;Feng Zhixin
猪肺炎支原体是引起猪持续性和继发性感染的重要呼吸道病原体。然而，这种情况发生的机制尚不清楚。在本研究中，我们建立了猪支气管上皮细胞(ALI-PBECs)的气液界面培养体系，该体系与活体内天然支气管的条件相似。我们利用该ALI-PBECs模型研究猪肺炎支原体在体外的感染和迁移特性。根据实验结果，我们证实猪肺炎分枝杆菌能够与ALI-PBECs黏附，并破坏粘液纤毛功能。重要的是，猪肺炎支原体可以通过细胞旁途径而不是跨细胞途径迁移到基底外侧小室，这是通过可逆地破坏紧密连接(TJ)和增加通透性并破坏上皮屏障的完整性来实现的。我们首次使用ALI-PBECs模型检测了猪肺炎支原体的迁移能力。上皮屏障的破坏使猪肺炎支原体通过细胞旁途径迁移到基底外侧腔，这可能与免疫逃避、肺外扩散和猪肺炎支原体的持续感染有关。
Mycoplasma hyopneumoniae is an important respiratory pathogen of pigs that causes persistent and secondary infections. However, the mechanisms by which this occurs are unclear. In this study, we established air-liquid interface culture systems for pig bronchial epithelial cells (ALI-PBECs) that were comparable to the conditions in the native bronchusin vivo. We used this ALI-PBECs model to study the infection and migration characteristics of M. hyopneumoniaein vitro. Based on the results, we confirmed that M. hyopneumoniae was able to adhere to ALI-PBECs and disrupt mucociliary function. Importantly, M. hyopneumoniae could migrate to the basolateral chamber through the paracellular route but not the transcellular pathway, and this was achieved by reversibly disrupting tight junctions (TJs) and increasing the permeability and damaging the integrity of the epithelial barrier. We examined the migration ability of M. hyopneumoniae using an ALI-PBECs model for the first time. The disruption of the epithelial barrier allowed M. hyopneumoniae to migrate to the basolateral chamber through the paracellular route, which may be related to immune evasion, extrapulmonary dissemination, and persistent infection of M. hyopneumoniae.