Genomic architecture of a genetically assimilated seasonal color pattern
Genomic architecture of a genetically assimilated seasonal color pattern
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DOI：
10.1126/science.aaz3017
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发表时间：
2020-11
期刊：
影响因子：
56.9
通讯作者：
Karin R. L. van der Burg;James J. Lewis;Benjamin J. Brack;R. A. Fandino;Anyi Mazo-Vargas;R. Reed
中科院分区：
文献类型：
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作者：
Karin R. L. van der Burg;James J. Lewis;Benjamin J. Brack;R. A. Fandino;Anyi Mazo-Vargas;R. Reed
常见的七叶树蝴蝶，Junonia coenia，表现出塑料着色;它有两种颜色变体，浅褐色和暗红色，这取决于白天的长度和温度。通过选择或多或少的颜色可塑性，货车德布尔格等人产生了蝴蝶品系，用于绘制差异着色的遗传变异。全基因组分析和RNA测序确定了最有可能与颜色可塑性差异相关的基因。用CRISPR-Cas9灭活基因鉴定了三个影响红色表型的基因，其他技术鉴定了与着色相关的顺式调节非编码基因组变体。从这些结果中，作者能够模拟遗传编码的可塑性和可塑性特征的同化可能如何进化。《科学》，本期第721页，基因组检查表征了季节性蝴蝶翅膀颜色背后的遗传机制。发育可塑性允许基因组编码多种不同的表型，这些表型可以在对环境线索的反应中表现出差异。替代塑料表型可以通过一种称为遗传同化的过程来选择，尽管其机制仍然知之甚少。我们同化了一个季节性的翅膀颜色表型在自然塑料种群的蝴蝶（Junonia coenia），其特征在于三个负责任的基因。内分泌测定和染色质的可及性和构象分析表明，过渡的翅膀着色从环境决定的性状，主要是遗传性状发生通过选择下游翼图案基因的调控等位基因。这种遗传进化模式可能受到选择的青睐，因为它允许对反应规范进行组织和性状特异性调整，而不影响核心线索检测或转导机制。
Untangling the genetics of plasticity The common buckeye butterfly, Junonia coenia, exhibits plastic coloration; it has two color morphs, light tan and dark red, that depend on day length and temperature. By selecting for more and less color plasticity, van der Burg et al. generated butterfly lines that were used to map the genetic variants that underlie differential coloration. Genome-wide analysis and RNA sequencing identified the genes most likely to be associated with the differences in color plasticity. Inactivation of genes with CRISPR–Cas9 identified three genes that affected the red phenotype, and other techniques identified cis-regulatory, noncoding genomic variants that were correlated with coloration. From these results, the authors were able to model how genetically encoded plasticity and assimilation of the plastic trait likely evolved. Science, this issue p. 721 A genomic examination characterizes the genetic mechanisms underlying a seasonal butterfly wing color. Developmental plasticity allows genomes to encode multiple distinct phenotypes that can be differentially manifested in response to environmental cues. Alternative plastic phenotypes can be selected through a process called genetic assimilation, although the mechanisms are still poorly understood. We assimilated a seasonal wing color phenotype in a naturally plastic population of butterflies (Junonia coenia) and characterized three responsible genes. Endocrine assays and chromatin accessibility and conformation analyses showed that the transition of wing coloration from an environmentally determined trait to a predominantly genetic trait occurred through selection for regulatory alleles of downstream wing-patterning genes. This mode of genetic evolution is likely favored by selection because it allows tissue- and trait-specific tuning of reaction norms without affecting core cue detection or transduction mechanisms.