Studies on comparative gel electrophoretic patterns of proteins and enzymes from isolates of Rhizoctonia solani causing sheath blight disease in rice
Studies on comparative gel electrophoretic patterns of proteins and enzymes from isolates of Rhizoctonia solani causing sheath blight disease in rice
复制标题
水稻纹枯病立枯丝核菌蛋白质和酶凝胶电泳图谱比较研究
DOI：
10.1139/m82-116
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发表时间：
1982
影响因子：
2.8
通讯作者：
K. Manibhushanrao
中科院分区：
文献类型：
--
作者：
M. Zuber;K. Manibhushanrao
用聚丙烯酰胺凝胶电泳技术对水稻纹枯病菌（Rhizoctoniasolani）5个不同致病力菌株的多酚氧化酶（DOPA）、过氧化物酶（POD）、乙酰酯酶（Acetylesterase）、酸性磷酸酶（Ac-PA）和碱性磷酸酶（Ac-PA）的蛋白质和同工酶谱进行了研究。虽然没有相关性，可以得到与酶的模式和菌株的毒力，两个菌株的毒力最低的菌丝和胞外过氧化物酶带，只有最低毒力的菌株给菌丝碱性磷酸酶带。
Protein and isoenzyme patterns of polyphenol (DOPA) oxidase, peroxidase, acetyl esterase, and acid and alkaline phosphatases from mycelial and extracellular extracts, as detected by polyacrylamide gel electrophoretic techniques, reflected marked variations among five differentially virulent isolates of Rhizoctonia solani, the causal organism of sheath blight disease of rice. Although no correlation could be obtained with the enzyme patterns and virulence of the isolates, two isolates with the lowest virulence showed mycelial and extracellular peroxidase bands and only the least virulent isolate gave mycelial alkaline phosphatase bands.