Viral interference between infectious hypodermal and hematopoietic necrosis virus and white spot syndrome virus in Litopenaeus vannamei
Viral interference between infectious hypodermal and hematopoietic necrosis virus and white spot syndrome virus in Litopenaeus vannamei
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DOI：
10.3354/dao072179
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发表时间：
2006-10-17
影响因子：
1.4
通讯作者：
Bonami, Jean-Robert
中科院分区：
文献类型：
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作者：
Bonnichon, Valerie;Lightner, Donald V.;Bonami, Jean-Robert
白斑综合征病毒（WSSV）是一种高毒力病毒，在过去十年中给虾养殖业造成了重大的生产损失。感染性皮下和造血坏死病毒（IHHNV）也感染对虾，虽然其重要性不如WSSV，但仍然是导致凡纳滨对虾（也称为凡纳滨对虾）和柱头对虾（也称为柱头对虾）显著产量损失的主要原因。这两种病毒及其相互作用先前在柱头对虾中进行了研究。我们在此报告了实验室攻击研究，以确定病毒在IHHNV和WSSV之间的干扰是否也发生在L. vannamei中，发现实验感染IHHNV可显著延迟WSSV攻击后的死亡率。分别于感染IHHNV后0、10、20、30、40和50 d用WSSV攻毒。单独感染WSSV的幼虾组在3 d内死亡，而预先感染IHHNV 30、40或50 d的幼虾组在5 d内死亡。实时荧光定量PCR分析显示，这种延迟与IHHNV载量相关，WSSV攻击导致IHHNV载量下降，表明两种病毒之间存在某种形式的竞争。
White spot syndrome virus (WSSV) is highly virulent and has caused significant production losses to the shrimp culture industry over the last decade. Infectious hypodermal and hematopoietic necrosis virus (IHHNV) also infects penaeid shrimp and, while being less important than WSSV, remains a major cause of significant production losses in Litopenaeus vannamei (also called Penaeus vannamei) and L. stylirostris (also called Penaeus stylirostris), These 2 viruses and their interactions were previously investigated in L. stylirostris. We report here laboratory challenge studies carried out to determine if viral interference between IHHNV and WSSV also occurs in L. vannamei, and it was found that experimental infection with IHHNV induced a significant delay in mortality following WSSV challenge. L. vannamei infected per os with IHHNV were challenged with WSSV at 0, 10, 20, 30, 40 and 50 d post-infection. Groups of naive shrimp infected with WSSV alone died in 3 d whereas shrimp pre-infected with IHHNV for 30, 40 or 50 d died in 5 d. Real-time PCR analysis showed that the delay correlated to the IHHNV load and that WSSV challenge induced a decrease in IHHNV load, indicating some form of competition between the 2 viruses.