Imaging of native high-density lipoprotein in human coronary plaques by color fluorescent angioscopy.
Imaging of native high-density lipoprotein in human coronary plaques by color fluorescent angioscopy.
复制标题
通过彩色荧光血管镜对人冠状动脉斑块中的天然高密度脂蛋白进行成像。
DOI：
10.1253/circj.cj-13-1585
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Y. Uchida
中科院分区：
文献类型：
--
作者：
Yasuto Uchida;N. Hiruta;Daisuke Yamanoi;Ei Shimoyama;Y. Maezawa;Y. Uchida
背景
高密度脂蛋白（HDL）在逆转胆固醇转运和阻止动脉粥样硬化的进展中发挥着关键作用。本研究的目的是可视化人类冠状动脉壁中的天然 HDL。
方法和结果
HDL 的荧光特性通过彩色荧光显微镜 (CFM) 进行研究，使用 470 nm 激发和 515 nm 发射，并以固绿染料 (FG) 作为生物标记物。通过彩色荧光血管镜检查 (CFA) 和 CFM 可视化 30 个正常冠状动脉节段以及切除的人冠状动脉中 25 个白色斑块和 25 个黄色斑块中的 HDL。将通过 CFM 可视化的 HDL 定位与使用抗 HDL 抗体进行免疫染色的定位进行比较。 FG 引发 HDL 特有的棕色荧光。通过CFA，正常节段、白色（斑块生长早期）和黄色（斑块生长晚期）斑块中HDL的发生率百分比分别为33%、76%（与正常节段和黄色斑块相比，P<0.05）和21%。 CFM 可视化的 HDL 定位与免疫染色的定位没有差异。
结论
在人冠状动脉壁中，HDL在正常节段很少沉积，但随着斑块形成而沉积增多，并在斑块生长晚期减少。
BACKGROUND
High-density lipoprotein (HDL) plays a key role in reverse cholesterol transport, and halts the progression of atherosclerosis. The aim of the present study was to visualize native HDL in the human coronary arterial wall.
METHODS AND RESULTS
The fluorescence characteristics of HDL were investigated by color fluorescent microscopy (CFM) using excitation at 470 nm and emission at 515 nm with Fast green dye (FG) as the biomarker. HDL in 30 normal coronary segments, and in 25 white and 25 yellow plaques in excised human coronary arteries, was visualized by color fluorescent angioscopy (CFA) and CFM. Localization of HDL visualized by CFM was compared with that stained by immunostaining using an anti-HDL antibody. FG elicited a characteristic brown fluorescence of HDL. By CFA, the percent incidence of HDL in normal segments, white (early stage of plaque growth) and yellow (advanced stage of plaque growth) plaques was, respectively, 33%, 76% (P<0.05 vs. normal segments and yellow plaques) and 21%. Localization of HDL visualized by CFM did not differ from that stained by immunostaining.
CONCLUSIONS
In the human coronary arterial wall, HDL deposits infrequently in normal segments, but increasingly deposits with plaque formation, and decreases in the advanced stage of plaque growth.